Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,877,005	(A)7→8	coding (97/348 nt)	yeaO →	conserved protein YeaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,998	1	.	A	100.0%	84.8 / NA	24	I30I (ATC→ATA)	yeaO	conserved protein YeaO
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (14/10);  total (14/10)

CAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATC‑‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCAC  >  NC_000913/1876923‑1877073                                                                             |                                                                             cAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATC‑‑aaaa                                                                         <  1:335992/80‑1 (MQ=255)          ttATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGAtt                                                                >  2:336029/1‑81 (MQ=255)           tATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGAtt                                                                >  1:336020/1‑80 (MQ=255)                  cGGCTTAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCtt                                                        <  2:335993/81‑1 (MQ=255)                  cGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCt                                                         <  1:335994/80‑1 (MQ=255)                         aCAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGt                                                 <  2:335995/81‑1 (MQ=255)                             agggATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAAAACCGATTTAGCCCTTGATGAGTggg                                              <  2:335996/78‑1 (MQ=255)                                 aTGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATa                                           >  1:336028/1‑79 (MQ=255)                                            aTACTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGcc                              >  2:336032/1‑81 (MQ=255)                                               cTGGTCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGt                            >  2:336035/1‑80 (MQ=255)                                                   tCGACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACg                       <  1:335997/81‑1 (MQ=255)                                                     gACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACgg                      >  1:336031/1‑80 (MQ=255)                                                     gACCGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACgg                      >  2:336030/1‑80 (MQ=255)                                                       ccGCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAAc                   <  1:335998/81‑1 (MQ=255)                                                         gCCTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTg                 <  1:335999/81‑1 (MQ=255)                                                          ccTCTGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgc                <  2:336000/81‑1 (MQ=255)                                                             ctGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgcgc              >  1:336038/1‑80 (MQ=255)                                                             ctGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgcgc              >  2:336036/1‑80 (MQ=255)                                                              tGGCCGCGCGGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgcgc              <  2:336001/79‑1 (MQ=255)                                                                  cgcgcgGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGcc        <  2:336002/81‑1 (MQ=255)                                                                      cgGTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCttt     >  1:336040/1‑80 (MQ=255)                                                                       ggTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCa   >  2:336025/1‑81 (MQ=255)                                                                       ggTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCa   >  1:336033/1‑81 (MQ=255)                                                                        gTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCAc  >  2:336027/1‑81 (MQ=255)                                                                        gTATCA‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCAc  >  1:336016/1‑81 (MQ=255)                                                                             |                                                                             CAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATC‑‑AAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTTTCAC  >  NC_000913/1876923‑1877073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑