Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,879,362	(T)8→9	intergenic (‑107/+41)	yeaQ ← / ← yoaG	PF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,879,354	1	.	T	100.0%	102.9 / NA	29	intergenic (‑99/+49)	yeaQ/yoaG	PF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/15);  total (14/15)

ATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTT  >  NC_000913/1879280‑1879429                                                                            |                                                                            aTTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑tttttt                                                                       >  2:336351/1‑81 (MQ=255)         gTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGAt                                                                <  2:336328/81‑1 (MQ=255)            aGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATgg                                                              <  1:336329/80‑1 (MQ=255)            aGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGgc                                                             >  2:336367/1‑81 (MQ=255)             gTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGgc                                                             <  2:336330/80‑1 (MQ=255)                gCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAg                                                         <  1:336331/81‑1 (MQ=255)                     tAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAAtt                                                    <  1:336332/81‑1 (MQ=255)                      aaaaaGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTg                                                   >  1:336356/1‑81 (MQ=255)                         aaGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTgcgc                                                <  2:336333/81‑1 (MQ=255)                            cATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCAt                                             >  1:336358/1‑81 (MQ=255)                            cATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCAt                                             >  2:336365/1‑81 (MQ=255)                                  acaacaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGa                                       >  1:336368/1‑81 (MQ=255)                                     acaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGAt                                      >  2:336360/1‑79 (MQ=255)                                     acaTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCa                                    <  2:336334/81‑1 (MQ=255)                                       aTCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGt                                  <  1:336335/81‑1 (MQ=255)                                           ggAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGAt                               >  2:336364/1‑80 (MQ=255)                                            gAGGGCAAGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATaa                             <  2:336336/81‑1 (MQ=255)                                                  aaGATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTacc                        >  1:336361/1‑80 (MQ=255)                                                    gATTTGTGACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTaccaac                     >  2:336366/1‑81 (MQ=255)                                                        tgtgACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACcac                  <  1:336337/80‑1 (MQ=255)                                                        tgtgACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACcac                  >  2:336373/1‑80 (MQ=255)                                                           gACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACaaa               >  2:336371/1‑80 (MQ=255)                                                           gACGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCACCAACGATCAGTGATAATTACCAACCACaaa               >  2:336376/1‑80 (MQ=255)                                                            aCGAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAc              <  1:336338/80‑1 (MQ=255)                                                              gAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAcatc           <  2:336339/81‑1 (MQ=255)                                                              gAGCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAcat            >  1:336378/1‑80 (MQ=255)                                                                gCATCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAAcatcat         <  1:336340/81‑1 (MQ=255)                                                                   tCACGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGtt      <  2:336341/81‑1 (MQ=255)                                                                     aCGGAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCg    >  1:336383/1‑81 (MQ=255)                                                                       ggAGGTTTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTAACAACCACAAACATCATGTTCGtt  <  1:336342/81‑1 (MQ=255)                                                                            |                                                                            ATTAGCGGGTTAGTCGCTGATAAAAAGCATAGCACAACATCGGGAGGGCAAGATTTGTGACGAGCATCACGGAGG‑TTTTTTTTGCGATGGCGCAGAAATTGCGCCATCAACGATCAGTGATAATTACCAACCACAAACATCATGTTCGTT  >  NC_000913/1879280‑1879429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑