Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,850	+C	intergenic (‑18/‑71)	sdiA ← / → sdsN	DNA‑binding transcriptional dual regulator SdiA/small regulatory RNA SdsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,846	1	.	C	100.0%	26.5 / NA	9	intergenic (‑14/‑75)	sdiA/sdsN	DNA‑binding transcriptional dual regulator SdiA/small regulatory RNA SdsN
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (1/8);  total (1/8)

CATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACG‑CCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACTATCACATAAATAA  >  NC_000913/1996773‑1996899                                                                           |                                                      cATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACG‑ccccctg                                                <  2:355380/81‑4 (MQ=255) cATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACG‑ccccct                                                 <  1:355381/80‑3 (MQ=255)       ctGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAgagt                                            >  2:355400/1‑80 (MQ=255)               gCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCAt                                   <  2:355382/81‑1 (MQ=255)                 aaCAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCAttt                                 <  2:355383/81‑1 (MQ=255)                               aCGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCa                   <  2:355384/81‑1 (MQ=255)                                  ccAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAAc                <  2:355385/81‑1 (MQ=255)                                   cAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACt               <  1:355386/81‑1 (MQ=255)                                     gCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACTAt             <  2:355387/81‑1 (MQ=255)                                          aaaaaTCCTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACTATCACat        <  2:355388/81‑1 (MQ=255)                                                ccTTATCCTGCATAGTAAACCGCAACGCCCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACTATCACataaataa  <  2:355389/81‑1 (MQ=255)                                                                           |                                                      CATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTGCATAGTAAACCGCAACG‑CCCCTGAGAGTGAGTATCATTTATAATGATAATTCAAACTATCACATAAATAA  >  NC_000913/1996773‑1996899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑