Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,201,740	(A)6→7	coding (1462/3633 nt)	yehI →	DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,201,734	1	.	A	100.0%	29.9 / NA	10	Q486K (CAA→AAA)	yehI	DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (3/7);  total (3/7)

CCAGGCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑AAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGGATGATGTATGGCTGTTTC  >  NC_000913/2201656‑2201794                                                                                |                                                             ccAGGCGAACAAGAGAGGCTTGCCGCCACTGGGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aa                                                            <  2:389386/81‑1 (MQ=255)   aGGCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaa                                                           >  1:389408/1‑80 (MQ=255)   aGGCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaa                                                           >  1:389416/1‑80 (MQ=255)    ggCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaaaa                                                         <  1:389387/81‑1 (MQ=255)    ggCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaaaa                                                         <  2:389388/81‑1 (MQ=255)       gAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaaaaaag                                                      >  1:389424/1‑79 (MQ=255)         aCAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaaaaaagtg                                                    >  2:389428/1‑77 (MQ=255)         aCAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑aaaaaaagtg                                                    >  1:389431/1‑77 (MQ=255)             gagagGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAGCGCATGAAACAAAAAAAGTGGACg                                                 <  2:389389/81‑1 (MQ=255)                      gccgccACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTAt                                         <  1:389390/80‑1 (MQ=255)                               gAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACt                               >  1:389427/1‑81 (MQ=255)                                      cccGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGtt                          <  2:389391/79‑1 (MQ=255)                                          ttATTTCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTggg                     >  1:389429/1‑80 (MQ=255)                                             tttCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGgatg                 <  2:389392/81‑1 (MQ=255)                                               tCAGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGgatga                <  1:402146/80‑1 (MQ=255)                                                 aGCACGGAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGGatgatg              >  1:389423/1‑80 (MQ=255)                                                       gAATCTCTTGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGGATGATGTATGGCt       <  1:389393/81‑1 (MQ=255)                                                              ttGCGCAACGCATGGAACAAAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGGATGATGTATGGCTGTTTc  <  1:389394/79‑1 (MQ=255)                                                                                |                                                             CCAGGCGAACAAGAGAGGCTTGCCGCCACTGAGCCTGCCCGTTATTTCAGCACGGAATCTCTTGCGCAACGCATGGAAC‑AAAAAAGTGGACGAGTTGTATTACAGGAACTGGGTTTTGGGGATGATGTATGGCTGTTTC  >  NC_000913/2201656‑2201794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑