| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,328,408 | (T)7→8 | coding (1390/1650 nt) | yfaQ ← | tandem DUF2300 domain‑containing protein YfaQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,328,401 | 1 | . | T | 96.3% | 90.9 / ‑4.3 | 27 | coding (1397/1650 nt) | yfaQ | tandem DUF2300 domain‑containing protein YfaQ |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (11/15); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑TTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAC > NC_000913/2328327‑2328476 | cTAAACGGC‑AACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tttt‑ttt < 1:412710/81‑1 (MQ=255) tAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tttt‑ttt > 1:412743/1‑81 (MQ=255) aaaCCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCgccagc < 2:412711/81‑1 (MQ=255) aCCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCgccagcca < 2:412712/81‑1 (MQ=255) aCCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCgccagc > 1:412735/1‑79 (MQ=255) aaCACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCttt < 2:412713/81‑1 (MQ=255) aaCACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCttt > 1:412749/1‑81 (MQ=255) acacGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTc < 2:412714/81‑1 (MQ=255) cacGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCg > 2:412737/1‑81 (MQ=255) cGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCt < 1:412715/81‑1 (MQ=255) cTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTg > 1:503386/1‑81 (MQ=255) ggTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTggg > 2:412744/1‑81 (MQ=255) gTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTgg < 2:412716/79‑1 (MQ=255) gTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACt > 1:412752/1‑81 (MQ=255) aCCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTgg > 1:412759/1‑81 (MQ=255) ccccGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGc < 2:412717/81‑1 (MQ=255) ccGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAg < 2:412718/81‑1 (MQ=255) cGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAgg > 1:412747/1‑81 (MQ=255) cAGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAg > 1:412751/1‑81 (MQ=255) aGCTTGTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGc > 2:412754/1‑81 (MQ=255) gTAATATACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTgg < 2:412719/80‑1 (MQ=255) atatACGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCt < 1:412720/81‑1 (MQ=255) aCGCCGCCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTccc > 2:412753/1‑80 (MQ=255) cgccgcCACTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCa < 1:412721/81‑1 (MQ=255) ccACTGCGGCATTTTT‑TTTCACCAGCCAGGCATTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGc < 2:412722/81‑1 (MQ=255) cACCGCGGCA‑TTTTCTTTCGCCAGCCACGCTTTCGCTTTGGGTAATAACTGTCAGTTTGTGCGTGCCGCTCCCCAGCGGc > 2:1027695/1‑80 (MQ=255) aCTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTa < 2:412723/81‑1 (MQ=255) cTGCGGCATTTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTaa < 2:412724/81‑1 (MQ=255) cGGCATTTTT‑TTTCGCCAGCCAGACTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAc < 2:412725/81‑1 (MQ=255) | CTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑TTTT‑TTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAC > NC_000913/2328327‑2328476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |