Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,403,762	+A	coding (84/1791 nt)	nuoC ←	NADH:quinone oxidoreductase subunit CD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,403,758	1	.	A	100.0%	83.7 / NA	24	G30W (GGG→TGG)	nuoC	NADH:quinone oxidoreductase subunit CD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (10/14);  total (10/14)

CCAGTAATTGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCC‑AAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCTTGCGCGGT  >  NC_000913/2403683‑2403833                                                                             |                                                                            ccAGTAATTGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCC‑aaaaa                                                                        >  1:427060/1‑80 (MQ=255)   aGTAATTGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCC‑aaaaacg                                                                      >  1:427061/1‑78 (MQ=255)       aTTGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGtt                                                                    <  1:427033/80‑1 (MQ=255)         tGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTgcg                                                                 >  2:427070/1‑81 (MQ=255)          gTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTgcg                                                                 >  1:427067/1‑80 (MQ=255)           ttCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCa                                                               <  1:427034/81‑1 (MQ=255)                        cacacaACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCAc                                                  >  2:427062/1‑81 (MQ=255)                              aCGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGatc                                            <  1:427035/81‑1 (MQ=255)                                  gAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGatcatc                                         >  2:427064/1‑80 (MQ=255)                                   aaCCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCaa                                       <  2:427036/81‑1 (MQ=255)                                       ccGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAgat                                    >  1:427037/1‑80 (MQ=255)                                         ggTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAgatgat                                 >  2:427071/1‑81 (MQ=255)                                         ggTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAgatgat                                 <  1:427038/81‑1 (MQ=255)                                             cGAGTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATcgc                              <  1:427039/80‑1 (MQ=255)                                                gTCGCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGt                          >  2:427065/1‑81 (MQ=255)                                                   gCCTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCt                        <  2:427040/80‑1 (MQ=255)                                                     cTGAACAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGcc                     <  2:427041/81‑1 (MQ=255)                                                        aaCAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAgg                  >  2:427068/1‑81 (MQ=255)                                                        aaCAGTAAAGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAg                   <  2:427042/80‑1 (MQ=255)                                                              aaaGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGtt            >  2:427078/1‑81 (MQ=255)                                                               aaGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTc           >  1:427077/1‑81 (MQ=255)                                                                aGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCt          <  2:427044/81‑1 (MQ=255)                                                                aGGCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCt          <  1:427043/81‑1 (MQ=255)                                                                 ggCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCtt         <  1:427045/81‑1 (MQ=255)                                                                 agCATCCGGCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCtt         <  1:427046/80‑1 (MQ=255)                                                                        ggCCCAAAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCTTGCGCGGt  <  1:427047/81‑1 (MQ=255)                                                                             |                                                                            CCAGTAATTGTTCACGCTTGATCCACACAACGGGAACCCCGGTGCGAGTCGCCTGAACAGTAAAGGCATCCGGCCC‑AAAACGGTTGCGCAGTTCGCCAATCACCGGATCATCAAGATGATCGCGGGTCTGCCAGGCGGGTTCTTGCGCGGT  >  NC_000913/2403683‑2403833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑