Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,449,154	+C	coding (549/552 nt)	smrB →	putative endonuclease SmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,449,151	1	.	C	100.0%	98.6 / NA	29	L182F (TTG→TTC)	smrB	putative endonuclease SmrB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (11/18);  total (11/18)

TCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTG‑CCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAG  >  NC_000913/2449073‑2449225                                                                                |                                                                           tcaGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTG‑cc                                                                          <  2:435767/81‑1 (MQ=255)        ccAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTgagg                                                                    <  1:435768/81‑1 (MQ=255)            aaGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGtt                                                                <  1:435769/81‑1 (MQ=255)                           aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCa                                                 <  2:435770/81‑1 (MQ=255)                                  gttgttGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACgg                                          >  1:435804/1‑81 (MQ=255)                                    tgttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACggg                                         >  1:435809/1‑80 (MQ=255)                                     gttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGt                                        <  2:435771/80‑1 (MQ=255)                                      ttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTa                                       <  1:435772/80‑1 (MQ=255)                                        ggTGTTGATTGAAGTCGAAGAGTGACTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAAt                                     <  2:435773/80‑1 (MQ=255)                                          tgtTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGc                                  <  2:435774/81‑1 (MQ=255)                                          tgtTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGc                                  <  2:435775/81‑1 (MQ=255)                                               attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAgggg                             >  2:435811/1‑81 (MQ=255)                                               attgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAggg                              <  2:435776/80‑1 (MQ=255)                                                ttgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGc                            <  2:435777/81‑1 (MQ=255)                                                    aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAAt                        <  2:435778/81‑1 (MQ=255)                                                     gTCGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATg                       >  2:435810/1‑81 (MQ=255)                                                       cGAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGcc                     >  2:435813/1‑81 (MQ=255)                                                        gAAGAGTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGccc                    >  1:435814/1‑81 (MQ=255)                                                          agagTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCcct                  >  1:435817/1‑81 (MQ=255)                                                          agagTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCcct                  <  1:435779/81‑1 (MQ=255)                                                            agTGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCcctgc                >  2:435822/1‑81 (MQ=255)                                                              tGGCTGCCGCCGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCcctgcct              <  1:435780/81‑1 (MQ=255)                                                                   gccgccGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGgtg          >  2:435829/1‑80 (MQ=255)                                                                   gccgccGGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGgtg          >  2:435823/1‑80 (MQ=255)                                                                     cgccgGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTAt       <  2:435781/81‑1 (MQ=255)                                                                     cgccgGAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTAt       <  1:435782/81‑1 (MQ=255)                                                                       ccgGAGTTGCCCCTGAGGAGTTGAGCGCGTTACGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTATCt     <  2:435783/81‑1 (MQ=255)                                                                         ggAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTATCt     >  1:435832/1‑79 (MQ=255)                                                                          gAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAg  <  2:435785/81‑1 (MQ=255)                                                                          gAGTTGCCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATAGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAg  <  2:435784/81‑1 (MQ=255)                                                                                |                                                                           TCAGGCACCAAAAGAGTATGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTG‑CCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAG  >  NC_000913/2449073‑2449225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑