Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,016	+A	coding (490/1017 nt)	eutD ←	phosphate acetyltransferase EutD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,013	1	.	A	100.0%	116.4 / NA	33	A165S (GCC→TCC)	eutD	phosphate acetyltransferase EutD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (15/18);  total (15/18)

GCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGC‑AAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGC  >  NC_000913/2572934‑2573086                                                                                 |                                                                          gcgcgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGc                                                                            >  2:458275/1‑80 (MQ=255) gcgcgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGc                                                                            <  1:458214/80‑1 (MQ=255) gcgcgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGC‑a                                                                          <  2:458215/81‑1 (MQ=255)   gcgcCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGC‑aaa                                                                        <  1:458216/81‑1 (MQ=255)              cGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGc                                                               <  2:458217/80‑1 (MQ=255)               ggCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGcc                                                              <  2:458218/80‑1 (MQ=255)                    tGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCg                                                        >  1:458273/1‑81 (MQ=255)                       cAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTg                                                     <  1:458219/81‑1 (MQ=255)                       cAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTg                                                     <  1:458220/81‑1 (MQ=255)                        aaGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGt                                                    <  2:458221/81‑1 (MQ=255)                          gcgcGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTAc                                                  <  1:458222/81‑1 (MQ=255)                           cgcgATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACt                                                 >  1:458265/1‑81 (MQ=255)                            gcgATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACtg                                                <  2:458223/81‑1 (MQ=255)                             cgATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACtgt                                               >  1:458261/1‑81 (MQ=255)                             cgATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACtgt                                               <  1:458224/81‑1 (MQ=255)                                tatCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTgg                                             >  2:458276/1‑80 (MQ=255)                                tatCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGc                                            <  2:458225/81‑1 (MQ=255)                                  tCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCaa                                          >  1:458272/1‑81 (MQ=255)                                   ccGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCaa                                          >  2:458278/1‑80 (MQ=255)                                     gCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACAt                                       >  2:458267/1‑81 (MQ=255)                                         gCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAgg                                   <  2:458226/81‑1 (MQ=255)                                          cTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAgg                                   >  1:458263/1‑80 (MQ=255)                                              gccgccGTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAAt                              >  2:458264/1‑81 (MQ=255)                                               ccgccgTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATg                             <  1:458227/81‑1 (MQ=255)                                                 gccgTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTATTGTGGCAACATCAGGAAAATgg                            >  2:458315/1‑80 (MQ=255)                                                    gTCGGCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGaa                          >  2:458270/1‑79 (MQ=255)                                                       ggCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAagaga                      <  1:458228/80‑1 (MQ=255)                                                        gCTGTGGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGc                    <  2:458229/81‑1 (MQ=255)                                                          tgtgGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGt                  >  2:458288/1‑81 (MQ=255)                                                            tgGCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATTAGGAAAATGGAAGAGAGCGtt                 <  2:458230/80‑1 (MQ=255)                                                              gCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGtttt               <  2:458232/80‑1 (MQ=255)                                                              gCACCACGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGtttt               <  1:458231/80‑1 (MQ=255)                                                                accacGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTa              >  2:458271/1‑79 (MQ=255)                                                                   acGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGcc         >  2:458266/1‑81 (MQ=255)                                                                    cGCTGCAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCt        <  2:458233/81‑1 (MQ=255)                                                                         cAATCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCt    <  2:458234/80‑1 (MQ=255)                                                                           aTCGGCAAAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGc  >  1:458274/1‑80 (MQ=255)                                                                                 |                                                                          GCGCGCCAGGTTTCGGCACTGGCAAGCGCGATATCCGCCAGCTGCGCCGCCGTCGGCTGTGGCACCACGCTGCAATCGGC‑AAAGCCCAACGCCGGGCCGCTGTACTGTGGCAACATCAGGAAAATGGAAGAGAGCGTTTTACAGCCTGGCTGC  >  NC_000913/2572934‑2573086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑