Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,592,892	(C)5→6	coding (131/201 nt)	ypfN →	UPF0370 protein YpfN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,592,887	1	.	C	100.0%	85.3 / NA	26	L42L (CTT→CTC)	ypfN	UPF0370 protein YpfN
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/9);  total (17/9)

GGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGT  >  NC_000913/2592809‑2592960                                                                                |                                                                          ggTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑cc                                                                         >  2:462450/1‑81 (MQ=255)  gTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑ccc                                                                        >  2:462487/1‑81 (MQ=255)  gTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑ccc                                                                        <  1:462433/81‑1 (MQ=255)  gTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑cc                                                                         <  2:462432/80‑1 (MQ=255)      gCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑ccccccg                                                                    >  1:462468/1‑79 (MQ=255)      gCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑ccccccg                                                                    >  1:462461/1‑79 (MQ=255)       cGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑ccccccgc                                                                   <  1:462434/81‑4 (MQ=255)               tgAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGAt                                                            >  1:462484/1‑81 (MQ=255)                gAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGAtt                                                           >  2:462470/1‑81 (MQ=255)                     tGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAAc                                                      >  2:462466/1‑81 (MQ=255)                           aaGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATaa                                                 <  1:462436/80‑1 (MQ=255)                                   aaGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTggg                                            <  1:462437/77‑1 (MQ=255)                                      cgcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATga                                     <  1:462438/81‑1 (MQ=255)                                       gcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgac                                    >  2:462471/1‑81 (MQ=255)                                        cgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgacg                                   <  2:462439/81‑1 (MQ=255)                                         gTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGAGATTTCAACGATAAGTGGGACGATgacga                                  >  1:462503/1‑81 (MQ=255)                                          tCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgacgac                                 <  1:462440/81‑1 (MQ=255)                                            gACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgacgacga                               >  2:462477/1‑81 (MQ=255)                                            gACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgacgacga                               >  1:462480/1‑81 (MQ=255)                                             aCCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATgacgacgac                              >  1:462486/1‑81 (MQ=255)                                              ccATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACt                             >  1:462472/1‑81 (MQ=255)                                              ccATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACt                             >  1:462497/1‑81 (MQ=255)                                               cATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTg                            <  1:462441/81‑1 (MQ=255)                                                         ttCTCGCCAACAAGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAg                  >  2:462491/1‑81 (MQ=255)                                                                caacaaGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAAc           >  1:462478/1‑81 (MQ=255)                                                                 aacaaGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAAcc          >  1:462479/1‑81 (MQ=255)                                                                 aacaaGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAAcc          >  2:462474/1‑81 (MQ=255)                                                                 aacaaGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAAcc          <  1:462442/81‑1 (MQ=255)                                                                   caaGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCga        <  2:462443/81‑1 (MQ=255)                                                                     aGCCGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCgaag      <  1:462444/81‑1 (MQ=255)                                                                        cGGAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGaagaag   >  1:462467/1‑81 (MQ=255)                                                                         ggAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGaagaag   >  1:462485/1‑80 (MQ=255)                                                                          gAGCTTCCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGt  >  1:462493/1‑80 (MQ=255)                                                                                |                                                                          GGTAGGCGTCCTGCTGAACGTGATTAAAGATCTCAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTT‑CCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGT  >  NC_000913/2592809‑2592960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑