| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,766,859 | (T)7→8 | coding (942/1074 nt) | rnlA → | CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,766,852 | 1 | . | T | 93.8% | 50.6 / ‑2.9 | 16 | coding (935/1074 nt) | rnlA | CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (5/10); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GCTCATTACATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑TTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTC > NC_000913/2766776‑2766921 | gctCATTACATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑tttt > 1:498322/1‑81 (MQ=255) aTTACATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑ttttttt < 1:498283/80‑1 (MQ=255) ttACATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑tttttttt > 2:498310/1‑79 (MQ=255) aCATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑ttttttttaat > 1:498313/1‑77 (MQ=255) ttCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAAtgtg < 1:498284/81‑1 (MQ=255) tCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAAtgtg > 2:498308/1‑80 (MQ=255) aaaCCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGa < 1:498285/78‑1 (MQ=255) aaCCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGt < 1:498286/81‑1 (MQ=255) cGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCAt < 1:498287/81‑1 (MQ=255) aaTTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑TTTTTTTAATGTGGAACGTCATtctc > 2:498311/1‑81 (MQ=255) tttGCAGCTACTCTTAGACCGGGACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTctct < 1:498289/81‑1 (MQ=255) tttGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTctct < 1:498288/81‑1 (MQ=255) cAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGtt < 1:498290/80‑1 (MQ=255) cAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTc > 1:498312/1‑81 (MQ=255) gACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACt > 1:498314/1‑81 (MQ=255) aCCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACtg > 1:498317/1‑81 (MQ=255) aaCATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCa < 2:498291/80‑1 (MQ=255) aaCATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCAg > 1:498324/1‑81 (MQ=255) aCATCATCAGCACAGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCAGc < 2:498292/81‑1 (MQ=255) acaGCCTATACTTTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTc < 1:498293/81‑1 (MQ=255) | GCTCATTACATTCTGAAACCGCAATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATAC‑TTTTTTTAATGTGGAACGTCATTCTCTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTC > NC_000913/2766776‑2766921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |