| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,947,713 | (T)7→8 | intergenic (+30/+44) | metV → / ← amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,947,706 | 1 | . | T | 95.0% | 64.7 / ‑3.0 | 20 | intergenic (+23/+51) | metV/amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (7/12); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
CTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCC > NC_000913/2947630‑2947781 | cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑tt‑tt > 1:536636/1‑81 (MQ=255)cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑tt‑tt < 2:536601/81‑1 (MQ=255) gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑tt‑ttt < 1:536602/79‑1 (MQ=255) tAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGt > 1:536630/1‑81 (MQ=255) aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGtct > 2:536638/1‑81 (MQ=255) aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCAt < 2:536603/81‑1 (MQ=255) gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATaaa < 2:536604/81‑1 (MQ=255) gtcgGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATaaaa > 2:536639/1‑79 (MQ=255) ggTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TTCTTTTTGTTTCTGGTCTCCCATAAAAAAgcg > 1:536642/1‑81 (MQ=255) tCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCa > 1:536653/1‑81 (MQ=255) cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgcg > 2:536640/1‑81 (MQ=255) cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgcg < 2:536605/81‑1 (MQ=255) cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgc < 1:536606/80‑1 (MQ=255) cccccGCAACCAATTATTGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCt < 2:536607/81‑1 (MQ=255) attGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCtt > 1:536645/1‑81 (MQ=255) ttGAACACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTtc < 1:536608/81‑1 (MQ=255) aaCACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTtctc < 2:536609/80‑1 (MQ=255) aaCACCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTtctc < 2:536610/80‑1 (MQ=255) acCCTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCa < 2:536611/81‑1 (MQ=255) cTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCg > 1:536644/1‑81 (MQ=255) cTAACGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCg < 1:536612/81‑1 (MQ=255) aCGGGTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTc < 1:536613/80‑1 (MQ=255) gggTGTTT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGcc < 2:536614/81‑1 (MQ=255) | CTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TT‑TTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCC > NC_000913/2947630‑2947781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |