Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,772	(T)8→9	intergenic (+126/‑78)	argP → / → scpA	DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,764	1	.	T	100.0%	99.6 / NA	28	intergenic (+118/‑86)	argP/scpA	DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (15/13);  total (15/13)

CTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC  >  NC_000913/3060694‑3060839                                                                        |                                                                            cttTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑tttttttttg                                                                   >  2:561625/1‑79 (MQ=255)          ttCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACa                                                           <  2:561582/81‑1 (MQ=255)            ccTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACaaa                                                         >  2:561618/1‑81 (MQ=255)             cTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAAc                                                        <  1:561583/81‑1 (MQ=255)                gAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAAcc                                                       <  1:561584/79‑1 (MQ=255)                gAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCa                                                     >  2:561604/1‑81 (MQ=255)                   tcacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACg                                                   >  2:561638/1‑80 (MQ=255)                    cacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGt                                                  >  1:561611/1‑80 (MQ=255)                     acatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTa                                                 >  1:561616/1‑80 (MQ=255)                          acaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAg                                            <  1:561585/80‑1 (MQ=255)                             aaaTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCa                                        <  1:561586/81‑1 (MQ=255)                               aTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCAcc                                      <  2:561587/81‑1 (MQ=255)                                tAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCg                                     <  2:561588/81‑1 (MQ=255)                                  gACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGt                                    <  1:561589/80‑1 (MQ=255)                                   aCAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAg                                  >  2:561624/1‑81 (MQ=255)                                     aaaTCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgc                                <  1:561591/81‑1 (MQ=255)                                     aaaTCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgc                                <  1:561590/81‑1 (MQ=255)                                         ctcAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCa                             >  1:561614/1‑80 (MQ=255)                                           cAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaaa                           >  2:561608/1‑80 (MQ=255)                                            aGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaa                            <  2:561592/78‑1 (MQ=255)                                            aGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATa                         >  2:561634/1‑81 (MQ=255)                                              gcggcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATAcc                       <  1:561593/81‑1 (MQ=255)                                                ggcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCt                     >  1:561629/1‑81 (MQ=255)                                                 gcAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTc                    <  1:561594/81‑1 (MQ=255)                                                  cAAAAAACGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCa                   >  1:561626/1‑81 (MQ=255)                                                   aaaaaaCGACGTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCAt                  >  2:561630/1‑81 (MQ=255)                                                          gacgTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGAtt           >  2:561621/1‑81 (MQ=255)                                                            cgTCTGAATGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGc         >  1:561620/1‑81 (MQ=255)                                                                   aTGCATTTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAc  >  2:561615/1‑81 (MQ=255)                                                                        |                                                                            CTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC  >  NC_000913/3060694‑3060839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑