Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,201	(T)6→7	coding (256/1278 nt)	yggP ←	putative zinc‑binding dehydrogenase YggP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,195	1	.	T	100.0%	70.7 / NA	21	G88S (GGC→AGC)	yggP	putative zinc‑binding dehydrogenase YggP
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (9/12);  total (9/12)

GCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGA  >  NC_000913/3077115‑3077266                                                                                  |                                                                        gccgccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGcc                                                                          >  2:565671/1‑81 (MQ=255)    gccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑ttt                                                                      <  2:565650/81‑1 (MQ=255)     ccAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑tttt                                                                     <  1:565651/81‑1 (MQ=255)         aaTATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑ttttttta                                                                 <  1:565652/81‑3 (MQ=255)             ttCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATAtt                                                              >  2:565688/1‑81 (MQ=255)                gTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGc                                                           <  1:565653/81‑1 (MQ=255)                   gCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCa                                                         <  2:565654/80‑1 (MQ=255)                   gCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCa                                                         <  1:565655/80‑1 (MQ=255)                       tAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTga                                                     <  1:565656/80‑1 (MQ=255)                         gCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTgaga                                                   <  2:565657/80‑1 (MQ=255)                           ccGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGAttt                                                <  2:565658/81‑1 (MQ=255)                                gAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTAcc                                           >  1:565690/1‑81 (MQ=255)                                 aaTATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTAcc                                           <  1:565659/80‑1 (MQ=255)                                   tatCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCAc                                        <  2:565660/81‑1 (MQ=255)                                       cGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTc                                     <  2:565661/80‑1 (MQ=255)                                            ggTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACaa                                <  2:565662/80‑1 (MQ=255)                                                   ccATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCg                        <  1:565663/81‑1 (MQ=255)                                                   ccATCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCg                        >  2:565693/1‑81 (MQ=255)                                                     aTCGCCGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCgg                       >  2:565738/1‑80 (MQ=255)                                                          cGGTTGCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACAtt                 >  1:565695/1‑81 (MQ=255)                                                               gCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGc            >  1:565696/1‑81 (MQ=255)                                                               gCAATACAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGc            >  2:565704/1‑81 (MQ=255)                                                                    aCAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCgg        >  2:565698/1‑80 (MQ=255)                                                                     cAAAACGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTa      <  1:565664/81‑1 (MQ=255)                                                                         aCGCTGGCCTTTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGa  >  1:565711/1‑81 (MQ=255)                                                                                  |                                                                        GCCGCCAAAATATTCGTAGCTGTAGCCCGCTGAATATCCGCTTGGTAACCCCATCGCCGGTTGCAATACAAAACGCTGGCC‑TTTTTTATATTTGCCAGTGAGATTTTTACCCACTTCGACAATAACCCCGGCACATTCATGCCCGGTAATGA  >  NC_000913/3077115‑3077266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑