| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 461,311 | (T)7→8 | intergenic (+69/‑140) | lon → / → hupB | Lon protease/DNA‑binding protein HU‑beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 461,304 | 1 | . | T | 95.2% | 69.6 / ‑3.6 | 21 | intergenic (+62/‑147) | lon/hupB | Lon protease/DNA‑binding protein HU‑beta |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (9/11); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑C‑TTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGGCTG > NC_000913/461227‑461376 | tgtgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑C‑ttt > 1:103255/1‑81 (MQ=255) tgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑C‑ttttt < 1:103231/81‑1 (MQ=255) aTAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGc > 2:103251/1‑81 (MQ=255) tAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCt < 2:103232/81‑1 (MQ=255) gTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTa < 2:103233/80‑1 (MQ=255) gcgcAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGAt < 1:103235/80‑1 (MQ=255) gcgcAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGAt < 2:103234/80‑1 (MQ=255) gcAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGc < 1:103237/81‑1 (MQ=255) gcAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGc < 2:103236/81‑1 (MQ=255) gcAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGc > 1:103259/1‑81 (MQ=255) aaTGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGAt < 1:103238/81‑1 (MQ=255) aTGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATc < 1:103239/81‑1 (MQ=255) aTGAACTAATAAAAACAGGGCTGGAAGGCTAATACGGGAATGCCAGCAG‑TTTTTTAGTCTCGATAAGTTAGATGGCGGAtg < 2:977415/81‑2 (MQ=255) cACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCgg < 1:103240/80‑1 (MQ=255) ataaAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCtt < 2:103241/79‑1 (MQ=255) ataaAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCtt < 2:103242/79‑1 (MQ=255) aaaaCAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCt > 1:103264/1‑81 (MQ=255) aaaaCAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCt > 1:103262/1‑81 (MQ=255) aaaCAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCt > 2:103265/1‑80 (MQ=255) aGGCTAATTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGtgtt > 1:103256/1‑81 (MQ=255) aaTTCGGGCTTGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAg > 1:103271/1‑81 (MQ=255) ttGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtggct > 1:103254/1‑81 (MQ=255) tGCCAGC‑CTTTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtggctg > 1:103261/1‑81 (MQ=255) | TGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGC‑C‑TTTTTTTGTCTCGCTAAGTTAGATGGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGGCTG > NC_000913/461227‑461376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |