Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,619	(T)6→7	coding (39/369 nt)	ybbC →	PF15631 family protein YbbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,613	1	.	T	100.0%	35.3 / NA	11	S11S (TCA→TCT)	ybbC	PF15631 family protein YbbC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/7);  total (4/7)

TTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAG  >  NC_000913/527556‑527681                                                           |                                                                     ttaGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGt                                                 <  1:117446/80‑1 (MQ=255)    gTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAAt                                              <  1:117447/80‑1 (MQ=255)        ggCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATgaga                                          >  1:117465/1‑80 (MQ=255)           cTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGc                                      <  1:117448/81‑1 (MQ=255)                  gagaTTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTAc                               <  1:117449/81‑1 (MQ=255)                  gagaTTCAATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTAc                               >  1:117462/1‑81 (MQ=255)                            gAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgat                      >  1:117467/1‑80 (MQ=255)                             aaaTATAGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCtgatga                    <  1:117450/81‑1 (MQ=255)                                   aGTTCAATATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACat              >  1:117464/1‑81 (MQ=255)                                        aaTATTTTCGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACattatt          <  1:117451/80‑1 (MQ=255)                                                cGATGCTTTCATTTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAg  <  1:117452/80‑1 (MQ=255)                                                           |                                                                     TTAGTGGGGCCTATAAGGAGATTCAATGAAATATAGTTCAATATTTTCGATGCTTTCA‑TTTTTTATACTATTTGCCTGTAATGAGACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAG  >  NC_000913/527556‑527681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑