| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,003,273 | 0 | T | C | 54.5% | ‑1.1 / 25.9 | 22 | I112V (ATC→GTC) | fliC | flagellar filament structural protein |
| Reads supporting (aligned to +/- strand): ref base T (8/2); new base C (9/3); total (17/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGA > NC_000913/2003197‑2003353 | aCGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATag > 1:356395/1‑79 (MQ=255)aCGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACag > 1:356403/1‑79 (MQ=255) cGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATagaag < 2:356377/81‑1 (MQ=255) ttCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGAc < 1:356378/81‑1 (MQ=255) cACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACagaaga > 2:356398/1‑78 (MQ=255) aCGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATagaaga > 2:356399/1‑77 (MQ=255) aCGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGa < 2:356379/81‑1 (MQ=255) tGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACt > 1:356411/1‑81 (MQ=255) aaCTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGATCAGACt > 1:356416/1‑79 (MQ=255) aGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCgg > 2:356408/1‑80 (MQ=255) aCGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTg > 1:356417/1‑81 (MQ=255) tCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTg > 2:356407/1‑80 (MQ=255) cAATTTCATCCAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTa > 1:356400/1‑81 (MQ=255) tttCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCg > 2:356422/1‑81 (MQ=255) ccAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGtt > 2:356415/1‑81 (MQ=255) ccAGACGGGATTTAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGtt > 1:356405/1‑81 (MQ=255) aGACGGGATTTAATTTCGTCCTGGATAGATGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCa > 2:356410/1‑81 (MQ=255) gggATTTAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGca > 1:356419/1‑81 (MQ=255) tttAATTTCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACg < 1:356380/81‑1 (MQ=255) tttCGTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGt > 2:356424/1‑80 (MQ=255) gTCCTGGACAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgtt < 1:356381/81‑1 (MQ=255) ccTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgttgt > 2:356443/1‑81 (MQ=255) tAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGa > 1:356412/1‑81 (MQ=255) | ACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGA > NC_000913/2003197‑2003353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |