Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,006,3200AG39.3% 19.7 / 25.2 28H20R (CAC→CGC) ygeWputative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base A (11/6);  new base G (5/6);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAA  >  NC_000913/3006240‑3006397
                                                                                |                                                                             
gTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCa                                                                               >  2:548786/1‑81 (MQ=255)
 tCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCa                                                                               <  1:548752/80‑1 (MQ=255)
      ccACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCGCgaga                                                                          <  2:548754/80‑1 (MQ=255)
      ccACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACgaga                                                                          <  1:548753/80‑1 (MQ=255)
           aCCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGAtt                                                                    >  1:548776/1‑81 (MQ=255)
             cGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGAtttt                                                                  >  2:548789/1‑81 (MQ=255)
                     tATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTaa                                                           <  2:548755/80‑1 (MQ=255)
                      atgatgAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACg                                                         >  1:548784/1‑81 (MQ=255)
                           gaAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTggg                                                     >  2:548788/1‑80 (MQ=255)
                            aaaaCTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGa                                                    <  2:548756/80‑1 (MQ=255)
                             gaaCTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAAc                                                  <  2:548757/80‑1 (MQ=255)
                              aaCTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGaa                                                   >  1:548778/1‑79 (MQ=255)
                              aaCTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGaa                                                   >  1:548781/1‑79 (MQ=255)
                               aCTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGaa                                                   >  2:548783/1‑78 (MQ=255)
                                  gTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACg                                             >  1:548794/1‑81 (MQ=255)
                                   ttAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGc                                            <  2:548758/81‑1 (MQ=255)
                                     aaTGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGcc                                           <  1:548759/80‑1 (MQ=255)
                                           cTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATaaa                                    >  1:548801/1‑81 (MQ=255)
                                              aTTAAGGATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACt                                  <  1:548760/80‑1 (MQ=255)
                                              aTTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACt                                  >  1:548806/1‑80 (MQ=255)
                                                    gATATCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAAc                             >  2:548799/1‑79 (MQ=255)
                                                     atatCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAg                          >  2:548804/1‑81 (MQ=255)
                                                       atCAATTCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTa                        >  1:548797/1‑81 (MQ=255)
                                                            ttCGCTGACCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGa                   <  2:548761/81‑1 (MQ=255)
                                                                cTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGtt               <  2:548762/81‑1 (MQ=255)
                                                                   aCCTCTCACCTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGcc            <  2:548763/81‑1 (MQ=255)
                                                                     ctctCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCgc          <  1:548764/81‑1 (MQ=255)
                                                                     ctctCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCg           >  1:548800/1‑80 (MQ=255)
                                                                           ccTTCGCGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCAt     >  2:548785/1‑80 (MQ=255)
                                                                              tCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTaa  >  1:548829/1‑80 (MQ=255)
                                                                                |                                                                             
GTCATTCCACTACCGGGACTTTATGATGAAAACTGTTAATGAGCTGATTAAGGATATCAATTCGCTGACCTCTCACCTTCACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAA  >  NC_000913/3006240‑3006397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑