| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,009,530 | 0 | A | G | 38.5% | 31.7 / 36.5 | 39 | D256G (GAC→GGC) | ygeY | putative peptidase YgeY |
| Reads supporting (aligned to +/- strand): ref base A (14/10); new base G (9/6); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGC > NC_000913/3009451‑3009605 | tATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGa > 2:549549/1‑80 (MQ=255) aTGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGAc > 2:549544/1‑80 (MQ=255) tGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAgcaggcag < 1:549490/81‑1 (MQ=255) gAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTgc < 1:549491/81‑1 (MQ=255) aaTTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTgcg > 1:549543/1‑81 (MQ=255) aTTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTgcgc < 2:549492/81‑1 (MQ=255) ttCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTgcg > 1:549548/1‑79 (MQ=255) cTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGtt > 1:549533/1‑81 (MQ=255) cTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACAGCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGt < 1:549493/80‑1 (MQ=255) ggCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCa < 1:549494/81‑1 (MQ=255) aaaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATc > 2:549547/1‑81 (MQ=255) aaaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATc > 1:549541/1‑81 (MQ=255) aaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCg < 1:549495/81‑1 (MQ=255) ggCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGAc > 2:549564/1‑81 (MQ=255) cACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACcg > 1:549552/1‑81 (MQ=255) cACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCt > 2:549540/1‑81 (MQ=255) cACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCt > 1:549546/1‑81 (MQ=255) ccGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGa < 1:549496/81‑1 (MQ=255) cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGAc > 2:549551/1‑81 (MQ=255) cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGAc > 1:549545/1‑81 (MQ=255) cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGAc < 1:549497/81‑1 (MQ=255) gTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGAcc > 1:549538/1‑81 (MQ=255) cTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTggg > 2:549554/1‑81 (MQ=255) aaaTCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCg < 2:549498/81‑1 (MQ=255) aaTCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCCGTTTCAATCGACCGTCGTCTCACCTgcggcg > 2:549562/1‑80 (MQ=255) aTCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGaa < 2:549499/81‑1 (MQ=255) cttcttCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAAc > 2:549557/1‑81 (MQ=255) tcACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTgg > 2:549580/1‑80 (MQ=255) acaTCCCCAAGCCGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGaa < 2:549500/81‑1 (MQ=255) acaTCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGaa < 2:549501/81‑1 (MQ=255) aaGCCGTTGCGCTGTAGCAGGCAGCTGCGCAATTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCt < 2:549502/81‑1 (MQ=255) cGTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc > 1:549573/1‑81 (MQ=255) cGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc < 1:549503/81‑1 (MQ=255) gTTGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACg < 2:549504/81‑1 (MQ=255) gTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc > 1:549558/1‑80 (MQ=255) gTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGAc > 2:549569/1‑80 (MQ=255) tGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGa > 2:549563/1‑80 (MQ=255) tGCGCTGTAGCAGGCAGCTGCGCAGTTTCAATCGACCGCCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGa > 1:549567/1‑80 (MQ=255) tAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcgc < 2:549506/80‑1 (MQ=255) tAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcgc < 2:549505/80‑1 (MQ=255) aGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCgcgc > 2:549550/1‑81 (MQ=255) | TATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGC > NC_000913/3009451‑3009605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |