Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,546,6030CT26.7% 43.8 / 17.2 30L15L (CTC→CTT) gntThigh‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base C (15/7);  new base T (3/5);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGC  >  NC_000913/3546523‑3546682
                                                                                |                                                                               
gACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTc                                                                                 >  2:681679/1‑81 (MQ=255)
gACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTc                                                                                 >  2:681667/1‑81 (MQ=255)
      aCCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCtgat                                                                            >  2:681666/1‑80 (MQ=255)
                aaTGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTc                                                                  >  1:681677/1‑80 (MQ=255)
                 aTGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCa                                                                 >  2:681686/1‑80 (MQ=255)
                  tGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCaa                                                                >  2:681662/1‑80 (MQ=255)
                        gCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAAc                                                         <  1:681628/81‑1 (MQ=255)
                            ggTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCt                                                     >  2:681688/1‑81 (MQ=255)
                             gTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCtt                                                    <  2:681629/81‑1 (MQ=255)
                              tAAATCATGCCATTAGTCATTGTTGCCATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTc                                                   <  2:681630/81‑1 (MQ=255)
                               aaaTCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCa                                                  >  1:681673/1‑81 (MQ=255)
                                aaTCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCTTCAt                                                 <  1:681631/81‑1 (MQ=255)
                                   cATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGc                                              >  2:681685/1‑81 (MQ=255)
                                    aTGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGct                                             >  2:681676/1‑81 (MQ=255)
                                       ccATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCtctc                                          >  1:681675/1‑81 (MQ=255)
                                             gTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCt                                     <  2:681633/80‑1 (MQ=255)
                                             gTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTc                                    <  1:681632/81‑1 (MQ=255)
                                                  tgttgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGgc                               >  1:681670/1‑81 (MQ=255)
                                                    ttgCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGgcgc                             >  1:681671/1‑81 (MQ=255)
                                                       cTATCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCtt                           <  2:681634/80‑1 (MQ=255)
                                                          tCGGTGTAATCTTGTTGTTGCTTCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCt                        <  2:681636/80‑1 (MQ=255)
                                                          tCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCt                        <  2:681635/80‑1 (MQ=255)
                                                          tCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTg                       >  1:681682/1‑81 (MQ=255)
                                                            ggTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGTCGCTTGCTGtt                     <  1:681637/81‑1 (MQ=255)
                                                             gtgtAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTg                    >  1:681711/1‑81 (MQ=255)
                                                             gtgtAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTg                    >  2:681691/1‑81 (MQ=255)
                                                                    cTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAAt             >  2:681687/1‑81 (MQ=255)
                                                                       gttgttgCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAAt             <  2:681638/78‑1 (MQ=255)
                                                                          gttgCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAg        >  1:681674/1‑80 (MQ=255)
                                                                             gCTTCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAAt    >  2:681680/1‑81 (MQ=255)
                                                                              cTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAAt    <  1:681639/80‑1 (MQ=255)
                                                                               tCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGc  >  1:681681/1‑81 (MQ=255)
                                                                                |                                                                               
GACGTTACCCATAACAAATGAAAGGCCAGGTAAATCATGCCATTAGTCATTGTTGCTATCGGTGTAATCTTGTTGTTGCTCCTGATGATCCGCTTCAAAATGAACGGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGC  >  NC_000913/3546523‑3546682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑