| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,145 | 0 | C | G | 20.6% | 83.5 / 10.9 | 34 | intergenic (+128/‑70) | lldD/trmL | L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (23/4); new base G (0/7); total (23/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACA > NC_000913/3781065‑3781224 | cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTCTTTTCTCCCTCGCCCCTCCGGGGAGAGGGcc > 2:742731/1‑81 (MQ=255) ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑gg < 1:742624/81‑1 (MQ=255) gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCTCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAg > 2:742673/1‑81 (MQ=255) gtatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAggg > 2:742641/1‑80 (MQ=255) cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGc > 1:742674/1‑81 (MQ=255) cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCg > 1:742700/1‑81 (MQ=255) ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCg > 1:742721/1‑80 (MQ=255) tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCAc > 2:742699/1‑81 (MQ=255) tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCAc > 2:742693/1‑81 (MQ=255) ctctGAAACGACACCGCACTC‑TTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCt > 1:742691/1‑81 (MQ=255) ccggAAACGACACCGCACTTTTTTTTTCCCCCCCCCCCCTCGGGGGAGAGGGCG‑GGGGTGGGGGG‑AAAAGGCCGCACTGCt < 2:742625/78‑1 (MQ=255) gaaaaCGACACCGCACTTTTTTTTTCCCCCCCGCCCCCCGGGGGAGGGGGCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTc < 2:742626/78‑1 (MQ=255) aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCAc > 1:742698/1‑80 (MQ=255) aCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCaa > 2:742689/1‑81 (MQ=255) acacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAttt > 2:742695/1‑81 (MQ=255) cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAAtttt > 2:742682/1‑81 (MQ=255) acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc > 1:742685/1‑81 (MQ=255) cGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg > 1:742670/1‑81 (MQ=255) gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc > 1:742688/1‑81 (MQ=255) gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc > 1:742681/1‑81 (MQ=255) ttttttCCCCCTCCCCCCTCCGGGGAG‑GGGCC‑GGGGGGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt < 2:742628/80‑1 (MQ=255) ttCCCCCCCGCCCCTCGGGGGGGAGGGCG‑GGGGGGAGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc < 2:742630/80‑1 (MQ=255) tctcCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc > 2:742686/1‑81 (MQ=255) cccgccccccGGGAGGGGGGCC‑CGGGCGGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt < 1:742643/77‑1 (MQ=255) gCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg > 1:742672/1‑81 (MQ=255) ggggAGGGGCCG‑GGGGTGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt < 1:742637/81‑1 (MQ=255) ggggAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTTTGCTATCCTGCCCCCGCACTAAGGGGGCAg > 2:742697/1‑80 (MQ=255) ggggAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg > 1:742706/1‑80 (MQ=255) ggggAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg > 1:742687/1‑80 (MQ=255) gggggggCG‑GGGGGGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg < 1:742631/77‑1 (MQ=255) gggagGGCG‑GGGGGGGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg < 1:742633/79‑1 (MQ=255) gagGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc > 1:742692/1‑80 (MQ=255) ggggCCGGGGGTGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt < 2:742632/80‑1 (MQ=255) agGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt > 1:742708/1‑80 (MQ=255) ggCG‑GGGGGGGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa < 1:742634/81‑1 (MQ=255) cc‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa > 2:742735/1‑81 (MQ=255) | CGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCC‑GGGGTGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACA > NC_000913/3781065‑3781224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |