Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,785,9840GC36.0% 58.3 / 55.8 50R242P (CGT→CCT) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (16/16);  new base C (9/9);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGT  >  NC_000913/3785906‑3786063
                                                                              |                                                                               
ccGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTg                                                                               >  2:744151/1‑81 (MQ=255)
  gATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCt                                                                             >  1:744133/1‑81 (MQ=255)
  gATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCt                                                                             >  1:744119/1‑81 (MQ=255)
    tACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGa                                                                           <  1:744057/81‑1 (MQ=255)
         ccgTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGt                                                                       >  1:744130/1‑80 (MQ=255)
             tGCTGGTTTGCCGTCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGTAAGCGACCGTTATCCGTGCTGAAGGTCAGc                                                                   >  2:744116/1‑80 (MQ=255)
              gCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGATAGCGACCGTTATCCGTGCTGAAGGTCAGc                                                                   >  2:744110/1‑79 (MQ=255)
              gCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGcc                                                                  <  1:744058/80‑1 (MQ=255)
              gCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGcc                                                                  <  2:744059/80‑1 (MQ=255)
               cTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAg                                                                <  1:744060/81‑1 (MQ=255)
                  gTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGAcg                                                             <  1:744061/81‑1 (MQ=255)
                   tttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAcgc                                                            <  2:744062/81‑1 (MQ=255)
                    ttGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGAcgcg                                                           >  2:744131/1‑81 (MQ=255)
                        aGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCaa                                                       <  2:744063/81‑1 (MQ=255)
                        aGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCaa                                                       >  2:744147/1‑81 (MQ=255)
                             gctTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATgg                                                    >  1:744146/1‑79 (MQ=255)
                              ctTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGaa                                                  <  2:744065/80‑1 (MQ=255)
                              ctTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAg                                                 <  2:744064/81‑1 (MQ=255)
                                tATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGAc                                               >  2:744136/1‑81 (MQ=255)
                                 aTGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACg                                              >  1:744156/1‑81 (MQ=255)
                                    cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCg                                           <  2:744066/81‑1 (MQ=255)
                                    cTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGc                                            <  2:744067/80‑1 (MQ=255)
                                       gcgACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATg                                        <  1:744068/81‑1 (MQ=255)
                                         gacgaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCa                                      >  1:744108/1‑81 (MQ=255)
                                          acgaAAATGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCAc                                     <  1:744069/81‑1 (MQ=255)
                                            gaAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTg                                   <  2:744070/81‑1 (MQ=255)
                                             aaaaTGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCAGCAATGGAAGACGGCGATGCACTGa                                  >  1:744139/1‑81 (MQ=255)
                                              aaaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAt                                 <  1:744071/81‑1 (MQ=255)
                                               aaTGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCGGACGCGGCAATGGAAGACGGCGATGCACTGAtt                                <  1:744072/81‑1 (MQ=255)
                                               aaTGATGAATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAtt                                >  1:744157/1‑81 (MQ=255)
                                                atgatgAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAttt                               <  1:744073/81‑1 (MQ=255)
                                                  gatgaATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGAtttt                              >  2:744138/1‑80 (MQ=255)
                                                   atgaATTCGTGAAAGCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTc                             <  1:744074/80‑1 (MQ=255)
                                                             gAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                  >  1:744144/1‑81 (MQ=255)
                                                              aaaGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCg                  <  2:744075/80‑1 (MQ=255)
                                                                aGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGc               >  2:744174/1‑81 (MQ=255)
                                                                 gCGACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCt              <  2:744076/81‑1 (MQ=255)
                                                                   gACCGTTATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGa            >  2:744150/1‑81 (MQ=255)
                                                                     ccGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAcc          <  2:744077/81‑1 (MQ=255)
                                                                     ccGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGAcc          <  2:744078/81‑1 (MQ=255)
                                                                       gTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc        >  2:744175/1‑81 (MQ=255)
                                                                       gTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc        >  2:744159/1‑81 (MQ=255)
                                                                       gTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc        >  1:744171/1‑81 (MQ=255)
                                                                       gTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgc        >  1:744126/1‑81 (MQ=255)
                                                                        ttATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcg       <  1:744080/81‑1 (MQ=255)
                                                                        ttATCCCTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcg       <  2:744079/81‑1 (MQ=255)
                                                                         tATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcgc      >  1:744143/1‑81 (MQ=255)
                                                                           tCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcgcgc    >  1:744166/1‑81 (MQ=255)
                                                                           tCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACcgcgcgc    <  1:744081/81‑1 (MQ=255)
                                                                             cGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGt  >  1:744141/1‑81 (MQ=255)
                                                                              gTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGt  >  2:744142/1‑80 (MQ=255)
                                                                              |                                                                               
CCGATACCGCCGTTGCTGGTTTGCAGGCTGCTTATGCTCGCGACGAAAATGATGAATTCGTGAAAGCGACCGTTATCCGTGCTGAAGGTCAGCCAGACGCGGCAATGGAAGACGGCGATGCACTGATTTTCATGAACTTCCGTGCTGACCGCGCGCGT  >  NC_000913/3785906‑3786063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑