Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,850,2600TC22.7% 83.9 / 20.4 44K156R (AAA→AGA) uhpADNA‑binding transcriptional activator UhpA
Reads supporting (aligned to +/- strand):  ref base T (14/20);  new base C (6/4);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGT  >  NC_000913/3850181‑3850340
                                                                               |                                                                                
gACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCttt                                                                                 >  1:760198/1‑81 (MQ=255)
 aCGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCttt                                                                                 <  2:760141/80‑1 (MQ=255)
   gCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCAc                                                                              >  2:760199/1‑81 (MQ=255)
    cccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCAcc                                                                             <  1:760142/81‑1 (MQ=255)
     ccAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCg                                                                            >  1:760189/1‑81 (MQ=255)
          ttttCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCACCGCCAtt                                                                       >  2:760216/1‑81 (MQ=255)
            ttCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTcc                                                                     >  2:760195/1‑81 (MQ=255)
               cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCtt                                                                   <  2:760144/80‑1 (MQ=255)
               cATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTg                                                                  <  1:760143/81‑1 (MQ=255)
                aTCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTgc                                                                 <  1:760145/81‑1 (MQ=255)
                 tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGTAATCTCTTTCACCGCCATTCCTTgcg                                                                <  2:760146/81‑1 (MQ=255)
                 tCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTgcg                                                                >  1:760227/1‑81 (MQ=255)
                  cAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTgcgc                                                               >  1:760191/1‑81 (MQ=255)
                   aGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTgcgc                                                               <  1:760147/80‑1 (MQ=255)
                   aGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGcc                                                              <  1:760148/81‑1 (MQ=255)
                     aTTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAg                                                            <  1:760149/81‑1 (MQ=255)
                        ggCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttt                                                         >  1:760211/1‑81 (MQ=255)
                        ggCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGttt                                                         >  1:760223/1‑81 (MQ=255)
                            cgATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTcc                                                     >  2:760202/1‑81 (MQ=255)
                            cgATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTcc                                                     <  2:760150/81‑1 (MQ=255)
                             gATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                    >  2:760212/1‑81 (MQ=255)
                             gATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCg                                                    >  2:760190/1‑81 (MQ=255)
                                 gACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAc                                                <  1:760152/81‑1 (MQ=255)
                                 gACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAc                                                <  2:760151/81‑1 (MQ=255)
                                  aCGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAcc                                               <  2:760153/81‑1 (MQ=255)
                                    gtgtACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTg                                             >  2:760208/1‑81 (MQ=255)
                                      gtACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGc                                           >  1:760220/1‑81 (MQ=255)
                                        aCCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGt                                         >  2:760213/1‑81 (MQ=255)
                                          cGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTc                                       <  1:760154/81‑1 (MQ=255)
                                            ttttCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCAc                                     >  1:760210/1‑81 (MQ=255)
                                                cGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGtt                                  <  2:760156/80‑1 (MQ=255)
                                                cGGTGACAAGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGtt                                  <  2:760155/80‑1 (MQ=255)
                                                       aaGCTCAGTTCGGCGGCAATCCCTTTCACCGCCATTCTTTGCGCCAGTCTTTCCGCTACCTGGCGTTCACGTTTGGTTAGc                          >  2:760197/1‑81 (MQ=255)
                                                       aaGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                          >  2:760230/1‑81 (MQ=255)
                                                       aaGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                          <  2:760158/81‑1 (MQ=255)
                                                       aaGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                          <  2:760157/81‑1 (MQ=255)
                                                       aaGCCCAGTTCGGCGGCAATCTCTCTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc                          >  1:760209/1‑81 (MQ=255)
                                                            cAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTc                     <  2:760160/81‑1 (MQ=255)
                                                            cAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTc                     <  2:760159/81‑1 (MQ=255)
                                                              gTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCt                   <  2:760161/81‑1 (MQ=255)
                                                                  ggcggcAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacg               <  1:760163/81‑1 (MQ=255)
                                                                  ggcggcAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgac                <  2:760162/80‑1 (MQ=255)
                                                                    cggcAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac             <  1:760164/81‑1 (MQ=255)
                                                                    cggcAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacgac             >  2:760222/1‑81 (MQ=255)
                                                                        aaTCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa         <  1:760165/81‑1 (MQ=255)
                                                                        aaTCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGa         >  2:760229/1‑81 (MQ=255)
                                                                              tttCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATg       <  2:760166/77‑1 (MQ=255)
                                                                               ttCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGt  >  1:760240/1‑81 (MQ=255)
                                                                               |                                                                                
GACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGT  >  NC_000913/3850181‑3850340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑