Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,455,0280CT28.1% 44.4 / 18.3 32M194I (ATG→ATA) fbpfructose‑1,6‑bisphosphatase 1
Reads supporting (aligned to +/- strand):  ref base C (17/6);  new base T (7/2);  total (24/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTACTTCTTCACCCCGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCGGTGGTGTA  >  NC_000913/4454950‑4455105
                                                                              |                                                                             
gTACTTCTTCACCCCGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCAt                                                                             >  2:921305/1‑81 (MQ=255)
         cACCCCGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTg                                                                    >  2:921299/1‑81 (MQ=255)
          aCCCCGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTgg                                                                   <  2:921244/81‑1 (MQ=255)
             ccGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGcac                                                                <  1:921245/81‑1 (MQ=255)
               gTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAg                                                              >  2:921283/1‑81 (MQ=255)
                  cGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAgg                                                             >  2:921295/1‑79 (MQ=255)
                    gAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAg                                                          >  2:921294/1‑80 (MQ=255)
                     aaaCTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGa                                                         >  2:921304/1‑80 (MQ=255)
                     aaaCTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGa                                                         >  2:921314/1‑80 (MQ=255)
                     aaaCTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGa                                                         >  2:921302/1‑80 (MQ=255)
                      aaCTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGaa                                                        >  2:921263/1‑80 (MQ=255)
                       aCTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGaaaa                                                      >  2:921303/1‑81 (MQ=255)
                        cTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAAc                                                     <  2:921246/81‑1 (MQ=255)
                          tAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGc                                                   >  2:921284/1‑81 (MQ=255)
                           aaTGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGcc                                                  >  1:921291/1‑81 (MQ=255)
                           aaTGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGcc                                                  >  1:921271/1‑81 (MQ=255)
                             tGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGa                                                <  1:921247/81‑1 (MQ=255)
                                aGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGc                                              >  1:921320/1‑80 (MQ=255)
                                aGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGc                                              >  2:921281/1‑80 (MQ=255)
                                   ttCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAg                                          >  1:921288/1‑81 (MQ=255)
                                        tCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCg                                     >  1:921296/1‑81 (MQ=255)
                                         cGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATc                                      <  2:921248/79‑1 (MQ=255)
                                             gATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCgtaag                                 >  1:921285/1‑80 (MQ=255)
                                               tGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCgtaagta                               >  2:921311/1‑80 (MQ=255)
                                                  aGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAgg                           <  2:921249/81‑1 (MQ=255)
                                                    tAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCg                         >  1:921326/1‑81 (MQ=255)
                                                        ttttGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGaa                     <  2:921250/81‑1 (MQ=255)
                                                          ttGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAaca                   >  2:921298/1‑81 (MQ=255)
                                                                ttCTCCGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCAt             >  1:921318/1‑81 (MQ=255)
                                                                    ccGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCgg         >  2:921319/1‑81 (MQ=255)
                                                                     cGGGAAGCGTATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCggt        >  2:921310/1‑81 (MQ=255)
                                                                      gggAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCggtg       <  2:921251/81‑1 (MQ=255)
                                                                           gcgtATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCGGTGgtgt   >  1:921308/1‑80 (MQ=255)
                                                                           gcgcATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCGGTGGTGTa  <  1:921252/81‑1 (MQ=255)
                                                                            cgcATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCGGTGGTGTa  >  1:921329/1‑80 (MQ=255)
                                                                              |                                                                             
GTACTTCTTCACCCCGTTCGGAAACTTAATGTAGTTTCCTTCGTTGATGGAGTAGGTTTTGCCTTTCTCCGGGAAGCGCATCCGTTCCTGGCACAGGCAGAAAACGCCGAGCGAAGGATCGTAAGTAAAGGCGTGAACACCGCATCCGGTGGTGTA  >  NC_000913/4454950‑4455105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑