| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 446,790 | 0 | G | A | 31.8% | 22.2 / 15.4 | 22 | intergenic (‑124/+25) | yajR/cyoE | putative major facilitator superfamily transport protein YajR/heme O synthase |
| Reads supporting (aligned to +/- strand): ref base G (12/3); new base A (4/3); total (16/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.17e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGA > NC_000913/446718‑446868 | gTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAACACCtttt < 2:100249/81‑1 (MQ=255) tAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCtttt > 2:100261/1‑80 (MQ=255) aaCATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAACACCTTTTTa < 2:100250/80‑1 (MQ=255) aCATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAACACCTTTTTaa > 1:100265/1‑80 (MQ=255) aTTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGt > 2:100272/1‑81 (MQ=255) ttAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGt > 2:100290/1‑81 (MQ=255) tAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTAcc < 1:100251/81‑1 (MQ=255) atatTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACcaca > 1:100258/1‑81 (MQ=255) atTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAACACCTTTTTAATAGAGAGGTTTTGTTACcaca > 1:100273/1‑79 (MQ=255) tGTTTCTAATGCTAAGAAAAAAGGTGCCGTAACACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTa < 1:100252/81‑1 (MQ=255) gTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTAt > 2:100271/1‑81 (MQ=255) tCTAATGCTAAGAAAAAAGGTGCCGTAACACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGc > 1:100285/1‑80 (MQ=255) tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTcc > 2:100309/1‑81 (MQ=255) tGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTcc < 2:100253/81‑1 (MQ=255) gCTAAGAAAAAAGGTGCCGTAACACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCg > 1:100284/1‑81 (MQ=255) aGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTAc < 1:100254/81‑1 (MQ=255) gCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCaa > 1:100283/1‑81 (MQ=255) gTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACg > 1:100281/1‑80 (MQ=255) gTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACg > 2:100282/1‑80 (MQ=255) gTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACg > 1:100274/1‑80 (MQ=255) aGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGa > 1:100295/1‑80 (MQ=255) aGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGa > 1:100292/1‑80 (MQ=255) | GTAAACATGCGGATTTTTTAGTAAATATTTCGAGAGGGATATGTTTCTAATGCTAAGAAAAAAGGTGCCGTAGCACCTTTTTAATAGAGAGGTTTTGTTACCACACAGCAGCCAGCAGCGTATGCGAGTCCGGTACCATAAAATCAACGGA > NC_000913/446718‑446868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |