Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 63,698 A→G Y695H (TAT→CAT)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091363,6980AG98.2% 183.5 / ‑4.2 55Y695H (TAT→CAT) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (30/24);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

CAAGGGGCGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGAT  >  NC_000913/63621‑63773
                                                                             |                                                                           
cAAGGGGCGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTc                                                                          <  2:15353/81‑1 (MQ=255)
 aaGGGGCGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCg                                                                         >  2:15405/1‑81 (MQ=255)
    gggCGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACGTGAGGCGGCACATTACGCTGATgctcgctc                                                                      <  1:15354/81‑1 (MQ=255)
       cGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGc                                                                   <  2:15355/81‑1 (MQ=255)
        gACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCg                                                                  >  2:15427/1‑81 (MQ=255)
         aCCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCg                                                                  >  2:15429/1‑80 (MQ=255)
          ccACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGa                                                                >  2:15407/1‑81 (MQ=255)
           cACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGAc                                                               >  2:15420/1‑81 (MQ=255)
            aCGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACg                                                              <  1:15356/81‑1 (MQ=255)
            aCGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACg                                                              <  1:15357/81‑1 (MQ=255)
             cGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACgg                                                             <  1:15358/81‑1 (MQ=255)
              gCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGTCGTCACATTACGCTGATGCTCGCTCAGCGGACGGc                                                            >  1:15415/1‑81 (MQ=255)
                  ttGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGaa                                                         <  1:15360/80‑1 (MQ=255)
                  ttGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGaa                                                         <  1:15361/80‑1 (MQ=255)
                  ttGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGa                                                          <  2:15359/79‑1 (MQ=255)
                   tGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAg                                                        <  1:15362/80‑1 (MQ=255)
                     gTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGc                                                      <  1:383922/80‑1 (MQ=255)
                         tcttcATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTa                                                 >  1:15417/1‑81 (MQ=255)
                          cttcATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTAc                                                >  2:15406/1‑81 (MQ=255)
                                 ggCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAAc                                         >  1:15425/1‑81 (MQ=255)
                                 ggCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAAc                                         >  2:15432/1‑81 (MQ=255)
                                   cAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAAc                                         >  2:15434/1‑79 (MQ=255)
                                    aaGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACTAg                                      >  2:15424/1‑81 (MQ=255)
                                    aaGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAg                                      <  1:15363/81‑1 (MQ=255)
                                         gAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGTCGAAGGCGTTTACGGTAAACCAGTcgcg                                 >  1:15437/1‑81 (MQ=255)
                                         gAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTcgcg                                 >  1:15451/1‑81 (MQ=255)
                                         gAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTcgcg                                 >  2:15433/1‑81 (MQ=255)
                                         gAGCGGCGCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTcacg                                 >  2:15419/1‑81 (MQ=255)
                                           gcggcgCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCa                               <  2:15364/81‑1 (MQ=255)
                                           gcggcgCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCa                               >  2:15428/1‑81 (MQ=255)
                                            cggcgCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCAt                              <  1:15365/81‑1 (MQ=255)
                                             ggcgCGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATc                             >  1:15423/1‑81 (MQ=255)
                                              gcgcGTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATc                             >  2:15452/1‑80 (MQ=255)
                                                gcgTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAg                          >  1:15462/1‑81 (MQ=255)
                                                 cgTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGt                         >  1:15402/1‑81 (MQ=255)
                                                 cgTACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGt                         <  1:15366/81‑1 (MQ=255)
                                                   tACATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGtt                        >  2:15421/1‑80 (MQ=255)
                                                     cATGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCAc                     >  2:15453/1‑81 (MQ=255)
                                                      aTGAGGCGGCACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCAcc                    >  1:15426/1‑81 (MQ=255)
                                                          ggcggcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGcc                <  1:15367/81‑1 (MQ=255)
                                                           gcggcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCa               <  2:15368/81‑1 (MQ=255)
                                                            cggcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCAt              <  1:15370/81‑1 (MQ=255)
                                                            cggcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCAt              <  1:15369/81‑1 (MQ=255)
                                                             ggcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATc             <  2:15371/81‑1 (MQ=255)
                                                              gcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCAt              >  1:15467/1‑79 (MQ=255)
                                                              gcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCAt              >  2:15435/1‑79 (MQ=255)
                                                              gcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCAt              >  2:15442/1‑79 (MQ=255)
                                                              gcACATTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCa            <  2:15372/81‑1 (MQ=255)
                                                                acaTTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGt          >  2:15441/1‑81 (MQ=255)
                                                                 caTTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGtt         >  2:15439/1‑81 (MQ=255)
                                                                  aTTACGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGttt        <  2:15373/81‑1 (MQ=255)
                                                                      cGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTc     <  2:15374/80‑1 (MQ=255)
                                                                      cGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTc     <  2:15375/80‑1 (MQ=255)
                                                                      cGCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTc     <  1:15376/80‑1 (MQ=255)
                                                                       gCTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGa   >  1:15447/1‑81 (MQ=255)
                                                                        cTGATGCTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGAt  <  2:15377/81‑1 (MQ=255)
                                                                             |                                                                           
CAAGGGGCGACCACGCTTTTGGTTTTCTTCATCGGCAAGGCGAGCGGCGCGTACATGAGGCGGCACATTACGCTGATACTCGCTCAGCGGACGGCGAAGGCGTTTACGGTAAACCAGTCGCGCATCCAGTTCACCCGCCATCAGTTTGTCGAT  >  NC_000913/63621‑63773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑