Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	610,876	C→T	R540H (CGC→CAC)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	610,876	0	C	T	100.0%	99.1 / NA	28	R540H (CGC→CAC)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

CAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATAGCAGCCG  >  NC_000913/610802‑610953                                                                           |                                                                              cAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTg                                                                          <  2:132858/80‑1 (MQ=255)    ccACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACt                                                                      >  2:132885/1‑81 (MQ=255)          aGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGAc                                                                >  2:132894/1‑81 (MQ=255)              cGCTTCAATCTTATTGCGATAATCGTTACGGAACTAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAAt                                                            >  1:132915/1‑81 (MQ=255)                     aTCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTttg                                                     <  2:132859/81‑1 (MQ=255)                            tacGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAATTCCAGACCAATCACTTTGTTGATGc                                              >  2:132903/3‑81 (MQ=255)                                  aaTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGttt                                        >  1:132907/1‑81 (MQ=255)                                   aTCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGttt                                        <  1:132860/80‑1 (MQ=255)                                    tCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCt                                      <  1:132861/81‑1 (MQ=255)                                     cGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTg                                     >  2:132900/1‑81 (MQ=255)                                      gTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGc                                    >  2:132890/1‑81 (MQ=255)                                       ttACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCt                                   >  2:132904/1‑81 (MQ=255)                                        tACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCtt                                  >  2:132896/1‑81 (MQ=255)                                         aCGGAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCtt                                  <  1:132862/80‑1 (MQ=255)                                           ggAACCAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCttt                                 >  1:132916/1‑79 (MQ=255)                                               ccAGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTc                           <  2:132863/81‑1 (MQ=255)                                                 aGGTGACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCAt                         >  1:132912/1‑81 (MQ=255)                                                     gACGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGt                      >  2:132899/1‑80 (MQ=255)                                                      aCGCCCGCCAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGtt                     <  1:132864/80‑1 (MQ=255)                                                          ccgccAGCCACCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCtt                <  2:132865/81‑1 (MQ=255)                                                            gccagccaCCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGc              <  1:132866/81‑1 (MQ=255)                                                                gccaCCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGa           >  2:132905/1‑80 (MQ=255)                                                                  caCCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATag        <  2:132867/81‑1 (MQ=255)                                                                   aCCCGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATagc       <  1:132868/81‑1 (MQ=255)                                                                    cccGTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATag        >  2:132906/1‑79 (MQ=255)                                                                       gTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATAGCAgcc   <  1:132870/81‑1 (MQ=255)                                                                       gTCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATAGCAgcc   <  1:132869/81‑1 (MQ=255)                                                                        tCGTGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATAGCAgccg  <  2:132871/81‑1 (MQ=255)                                                                           |                                                                              CAGCCACATAGCCCGCTTCAATCTTATTGCGATAATCGTTACGGAACCAGGTGACGCCCGCCAGCCACCCGTCGCGTTTGAACTCCAGACCAATCTCTTTGTTGATGCTGGTTTCTGCTTTCAGGTCATCGTTACCTTGCAGATAGCAGCCG  >  NC_000913/610802‑610953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑