Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	612,345	A→G	V50V (GTT→GTC)	fepA ←	ferric enterobactin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	612,345	0	A	G	100.0%	78.8 / NA	24	V50V (GTT→GTC)	fepA	ferric enterobactin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

CTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGG  >  NC_000913/612269‑612422                                                                             |                                                                              cTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGc                                                                           <  1:133166/81‑1 (MQ=255)         gTACGGATGATCTTCGACACATCGCGGGCAACCGTGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGcc                                                                   <  2:133167/81‑1 (MQ=255)              gatgatCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGt                                                                >  2:133197/1‑79 (MQ=255)                       cGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCt                                                         <  1:133168/77‑1 (MQ=255)                       cGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCt                                                         <  2:133169/77‑1 (MQ=255)                          cacaTCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcggc                                                  <  2:133170/81‑1 (MQ=255)                           acaTCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcggcg                                                 <  2:133171/81‑1 (MQ=255)                              tCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTcggcggcg                                              <  2:133172/81‑1 (MQ=255)                                gcgGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGt                                            <  2:133173/81‑1 (MQ=255)                                 cgGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGt                                            >  2:133208/1‑80 (MQ=255)                                  gggCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTaa                                          >  1:133203/1‑81 (MQ=255)                                   ggCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAAc                                         >  1:133215/1‑81 (MQ=255)                                         cGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACaa                                    >  1:133216/1‑80 (MQ=255)                                                  gCGGATTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGt                           >  1:133211/1‑80 (MQ=255)                                                      aTTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCAtgt                      <  2:133174/81‑1 (MQ=255)                                                      aTTTCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCAtgt                      >  1:133210/1‑81 (MQ=255)                                                         tCATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGa                    >  2:133217/1‑80 (MQ=255)                                                          cATCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGaaa                  >  2:133214/1‑81 (MQ=255)                                                           aTCTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAAc                 <  2:133175/81‑1 (MQ=255)                                                             cTGCGGTGATGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAg               >  2:133207/1‑81 (MQ=255)                                                                     aTGGTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtc        <  1:133176/80‑1 (MQ=255)                                                                        gTCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtc    <  1:133177/81‑1 (MQ=255)                                                                         tCGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtcg   <  2:133178/81‑1 (MQ=255)                                                                          cGAGACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTAtcggtcgg  >  2:133209/1‑81 (MQ=255)                                                                             |                                                                              CTGGCATGGTACGGATGATCTTCGACACATCGCGGGCAACCGGGTTTTTGCGGATTTCATCTGCGGTGATGGTCGAAACGCCAGGCGCCTGTAAGTTCTGCTCGGCGGCGGTAACGACAATAGTATCGTCATGTGAAACAGGAGTATCGGTCGG  >  NC_000913/612269‑612422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑