Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	633,421	T→C	P19P (CCA→CCG)	hcxA ←	hydroxycarboxylate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	633,421	0	T	C	100.0%	91.2 / NA	27	P19P (CCA→CCG)	hcxA	hydroxycarboxylate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

CGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTG  >  NC_000913/633345‑633495                                                                             |                                                                           cgcgTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGAt                                                                        <  1:137840/81‑1 (MQ=255)     tttGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGAtgtg                                                                     >  1:137876/1‑80 (MQ=255)      ttGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGa                                                                    >  1:137881/1‑80 (MQ=255)         ccGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGaaaa                                                                 <  2:137842/80‑1 (MQ=255)         ccGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAag                                                                <  2:137841/81‑1 (MQ=255)          cGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAagt                                                               <  1:137843/81‑1 (MQ=255)            tAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAagtag                                                             <  1:137844/81‑1 (MQ=255)              gATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAagtagt                                                            <  2:137845/80‑1 (MQ=255)              gATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGtt                                                           <  2:137846/81‑1 (MQ=255)               aTCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAagtagt                                                            >  1:137888/1‑79 (MQ=255)                tCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAg                                                         >  2:137896/1‑81 (MQ=255)                       cgcgcgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGcc                                                  <  1:137847/81‑1 (MQ=255)                          gcgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgac                                               <  2:137848/81‑1 (MQ=255)                          gcgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgac                                               >  2:137910/1‑81 (MQ=255)                           cgAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgac                                               >  1:137897/1‑80 (MQ=255)                            gagaAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCcgacga                                             >  2:137887/1‑81 (MQ=255)                             agaAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCgacgac                                            >  1:137898/1‑81 (MQ=255)                                   ttgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCAcgcg                                      <  2:137849/81‑1 (MQ=255)                                                 aaaaaTCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAg                        <  2:137850/81‑1 (MQ=255)                                                    aaTCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCa                     >  2:137885/1‑81 (MQ=255)                                                     aTCGTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAt                    <  1:137851/81‑1 (MQ=255)                                                        gTGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAtgtt                 <  2:137852/81‑1 (MQ=255)                                                         tGCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCCtgttg                >  2:137902/1‑81 (MQ=255)                                                          gCAGGTGATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCAtgttgt               >  1:137890/1‑81 (MQ=255)                                                                gATTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTc         >  2:137886/1‑81 (MQ=255)                                                                 aTTGAAACTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTc         <  2:137853/80‑1 (MQ=255)                                                                       gcTTCCCGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTg  <  1:137854/80‑1 (MQ=255)                                                                             |                                                                           CGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTG  >  NC_000913/633345‑633495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑