Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	653,615	T→C	F461L (TTC→CTC)	dpiB →	sensory histidine kinase DpiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	653,615	0	T	C	100.0%	116.8 / NA	34	F461L (TTC→CTC)	dpiB	sensory histidine kinase DpiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/19);  total (15/19)

GCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTACGAGAC  >  NC_000913/653537‑653692                                                                               |                                                                              gCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTc                                                                             <  2:141563/81‑1 (MQ=255)   cATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCt                                                                           >  1:141623/1‑81 (MQ=255)    aTTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCt                                                                           >  1:141604/1‑80 (MQ=255)          ggCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGa                                                                     <  2:141564/80‑1 (MQ=255)             aaTTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGaa                                                                 <  1:141565/81‑1 (MQ=255)                  aCTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCg                                                             <  2:141566/80‑1 (MQ=255)                    ttGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCgat                                                           >  1:141611/1‑80 (MQ=255)                       aTAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGatgatg                                                       <  1:141567/81‑1 (MQ=255)                          aCGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATgtgg                                                    <  1:141568/81‑1 (MQ=255)                           cGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATgtggt                                                   <  1:141569/81‑1 (MQ=255)                            gCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATgtggt                                                   >  1:141610/1‑80 (MQ=255)                            gCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATgtggt                                                   >  2:141634/1‑80 (MQ=255)                            gCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATgtggt                                                   >  1:141594/1‑80 (MQ=255)                              cTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGAt                                                <  2:141570/81‑1 (MQ=255)                                tCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGAtt                                               >  1:141593/1‑80 (MQ=255)                                  gAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTg                                              >  1:141622/1‑79 (MQ=255)                                     gccagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTc                                         >  2:141626/1‑81 (MQ=255)                                     gccagccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTc                                         >  1:141618/1‑81 (MQ=255)                                        agccTGCGTAGCGATGAAGGAAACAAGATCGTTGTATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGcc                                      >  2:141621/1‑81 (MQ=255)                                        agccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGcc                                      <  2:141571/81‑1 (MQ=255)                                          ccTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGa                                    >  1:141627/1‑81 (MQ=255)                                           cTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGAt                                   <  2:141572/81‑1 (MQ=255)                                              cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATc                                  <  1:141573/79‑1 (MQ=255)                                              cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATc                                  <  2:141575/79‑1 (MQ=255)                                              cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCa                                 <  2:141576/80‑1 (MQ=255)                                              cGTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGACGATGATGTGGTGATTGAAGTCGCCGATc                                  <  2:141574/79‑1 (MQ=255)                                               gTAGCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAgg                               >  2:141620/1‑81 (MQ=255)                                                  gCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGc                             <  1:141578/80‑1 (MQ=255)                                                  gCGATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCt                            <  2:141577/81‑1 (MQ=255)                                                    gATGAAGGAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTgc                          >  1:141613/1‑81 (MQ=255)                                                          ggAAACAAGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGtt                    <  2:141579/81‑1 (MQ=255)                                                                aaGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCagag              <  2:141580/81‑1 (MQ=255)                                                                aaGATCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCagag              <  2:400651/81‑1 (MQ=255)                                                                   aTCGTTGAATTACTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGtct           >  1:141624/1‑81 (MQ=255)                                                                           attactCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTACgaga   <  1:141581/81‑1 (MQ=255)                                                                            ttactCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTACGAGAc  <  2:141582/81‑1 (MQ=255)                                                                               |                                                                              GCCATTGTGGGCAATTTACTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTATTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTTCCAGAGTCTCTACGAGAC  >  NC_000913/653537‑653692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑