Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	654,745	C→T	S408S (TCG→TCA)	dcuC ←	anaerobic C4‑dicarboxylate transporter DcuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	654,745	0	C	T	100.0%	140.1 / NA	39	S408S (TCG→TCA)	dcuC	anaerobic C4‑dicarboxylate transporter DcuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/20);  total (19/20)

GGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGC  >  NC_000913/654673‑654822                                                                         |                                                                              ggTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGt                                                                       <  2:141789/81‑1 (MQ=255)    gCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACg                                                                    >  2:141843/1‑81 (MQ=255)     cGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACgg                                                                   <  1:141790/81‑1 (MQ=255)     cGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACg                                                                    <  1:141791/80‑1 (MQ=255)       ttttACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGcc                                                                 <  1:141792/81‑1 (MQ=255)         ttACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGcc                                                                 >  1:141833/1‑79 (MQ=255)            cGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGGAACGGGTGAAAGGGTACGGCCAAGGt                                                            >  2:141838/1‑81 (MQ=255)            cGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGt                                                            >  2:141845/1‑81 (MQ=255)               cTTCAAACGGCGAGATCTTCGCCATCCCGGCGACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTg                                                         <  2:141794/81‑1 (MQ=255)               cTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGttt                                                          >  2:141828/1‑80 (MQ=255)               cTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTg                                                         <  2:141793/81‑1 (MQ=255)                ttCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGa                                                        >  1:141827/1‑81 (MQ=255)                ttCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGa                                                        >  1:141830/1‑81 (MQ=255)                ttCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGa                                                        >  1:141846/1‑81 (MQ=255)                  cAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGAc                                                       <  1:141795/80‑1 (MQ=255)                    aaCGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGcc                                                    <  2:141796/81‑1 (MQ=255)                      cGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTg                                                  >  2:141818/1‑81 (MQ=255)                          gagaTCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTgcagc                                              <  2:141797/81‑1 (MQ=255)                            gaTCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCAt                                            <  2:141798/81‑1 (MQ=255)                            gaTCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCAt                                            <  1:141799/81‑1 (MQ=255)                                ttCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCgg                                         >  1:141820/1‑80 (MQ=255)                                  cGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGa                                       <  1:141800/80‑1 (MQ=255)                                   gCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGa                                       >  1:141844/1‑79 (MQ=255)                                   gCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGAt                                      <  1:141801/80‑1 (MQ=255)                                     cATCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGt                                   <  1:141802/81‑1 (MQ=255)                                      aTCCCGGCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGt                                   >  2:141835/1‑80 (MQ=255)                                            gCAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATAc                            <  2:141803/81‑1 (MQ=255)                                             cAACCGCAACGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACg                           >  2:141829/1‑81 (MQ=255)                                                    aaCGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGtt                    <  2:141804/81‑1 (MQ=255)                                                    aaCGACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGt                     >  2:141850/1‑80 (MQ=255)                                                       gACTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAAt                 >  2:141842/1‑81 (MQ=255)                                                        aCTACGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATg                <  2:141805/81‑1 (MQ=255)                                                            cGCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCgg            <  2:141806/81‑1 (MQ=255)                                                             gCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCgg            >  1:141834/1‑80 (MQ=255)                                                             gCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCgg            >  2:141848/1‑80 (MQ=255)                                                             gCCAGAAACGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGa           <  2:141807/81‑1 (MQ=255)                                                                    aCGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgc    >  2:141840/1‑81 (MQ=255)                                                                    aCGGGTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgc    <  1:141808/81‑1 (MQ=255)                                                                      gggTGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTgcgc  >  1:141839/1‑81 (MQ=255)                                                                         |                                                                              GGTGCGTTTTACGACTTCAAACGGCGAGATCTTCGCCATCCCGGCAACCGCAACGACTACGCCAGAAACGGGCGAAAGGGTACGGCCAAGGTTTGACGCCTGCAGCATCGGGATAGTCAAATACGCCGGGTTAATGCCGGAAGAGTGCGC  >  NC_000913/654673‑654822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑