Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,699	G→A	T173T (ACC→ACT)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,699	0	G	A	100.0%	129.4 / NA	36	T173T (ACC→ACT)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

ATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGAC  >  NC_000913/667621‑667776                                                                               |                                                                              aTAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGt                                                                             >  1:144126/1‑81 (MQ=255)      tGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACg                                                                        >  1:144143/1‑81 (MQ=255)            gTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAAc                                                                  >  1:144154/1‑81 (MQ=255)               tGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCg                                                                <  1:144100/80‑1 (MQ=255)                      attattCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGAt                                                         <  1:144101/80‑1 (MQ=255)                       ttattCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAg                                                       >  2:144161/1‑81 (MQ=255)                        tattCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAg                                                       >  1:144163/1‑80 (MQ=255)                         attCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGt                                                      >  1:144156/1‑80 (MQ=255)                          ttCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTa                                                     <  1:144102/80‑1 (MQ=255)                          ttCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTa                                                     <  2:144103/80‑1 (MQ=255)                              ggCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAacgac                                                >  2:144147/1‑81 (MQ=255)                              ggCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAacgac                                                <  2:144104/81‑1 (MQ=255)                                cGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGt                                              <  1:144106/81‑1 (MQ=255)                                cGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGt                                              <  1:144105/81‑1 (MQ=255)                                    cGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTta                                           >  1:144150/1‑80 (MQ=255)                                     gACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTta                                           >  1:144167/1‑79 (MQ=255)                                      aCGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAg                                        <  1:144107/81‑1 (MQ=255)                                       cGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGc                                       <  2:144108/81‑1 (MQ=255)                                       cGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGc                                       >  1:144152/1‑81 (MQ=255)                                          cTTTATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCtt                                    >  1:144160/1‑81 (MQ=255)                                             tATCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTaa                                 >  1:144164/1‑81 (MQ=255)                                               tCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAAc                                <  1:144109/80‑1 (MQ=255)                                               tCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACt                               <  2:144110/81‑1 (MQ=255)                                               tCGTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACt                               <  1:144111/81‑1 (MQ=255)                                                 gTTGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTc                             >  1:144142/1‑81 (MQ=255)                                                   tGATTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGa                           >  2:144158/1‑81 (MQ=255)                                                     aTTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGaca                         <  2:144113/81‑1 (MQ=255)                                                     aTTTTCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGac                          <  1:144112/80‑1 (MQ=255)                                                      ttttCGACACATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGacac                        >  1:144157/1‑81 (MQ=255)                                                            acacATAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGaa                  <  1:144114/81‑1 (MQ=255)                                                                aTAGCCGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACg              <  2:144115/81‑1 (MQ=255)                                                                    ccGATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTAc          <  2:144117/81‑1 (MQ=255)                                                                    ccGATGACGTGAGTCAACGCCGAACCGTAAGAATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTAc          <  2:144116/81‑1 (MQ=255)                                                                      gATGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACt         >  2:144155/1‑80 (MQ=255)                                                                       aTGACGTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGa       <  1:144118/81‑1 (MQ=255)                                                                            gTGAGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGAc  <  2:144119/81‑1 (MQ=255)                                                                               |                                                                              ATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGAC  >  NC_000913/667621‑667776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑