Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,641	A→G	L294S (TTA→TCA)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,641	0	A	G	100.0%	168.9 / NA	48	L294S (TTA→TCA)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/27);  total (21/27)

CCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGC  >  NC_000913/73564‑73717                                                                              |                                                                             ccGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGaca                                                                           >  1:18031/1‑81 (MQ=255) ccGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGac                                                                            <  1:17968/80‑1 (MQ=255)   ggCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACAcc                                                                         <  2:17969/81‑1 (MQ=255)        cTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTc                                                                    <  2:17970/81‑1 (MQ=255)          ggCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCg                                                                   >  2:18016/1‑80 (MQ=255)             gATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCaaa                                                               >  2:18032/1‑81 (MQ=255)              aTTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAAt                                                              <  1:17971/81‑1 (MQ=255)                 tACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGc                                                            <  1:17972/80‑1 (MQ=255)                  aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCgg                                                          >  2:18023/1‑81 (MQ=255)                  aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCg                                                           <  2:17973/80‑1 (MQ=255)                    cccATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCt                                                        <  1:17974/81‑1 (MQ=255)                      cATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTAt                                                      >  1:18055/1‑81 (MQ=255)                      cATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTAt                                                      >  2:18040/1‑81 (MQ=255)                         cgacgaTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACTATTGACACCGTGTCGCAAATGCGGCTATg                                                     >  2:18028/1‑79 (MQ=255)                                 aCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATc                                            <  2:17975/80‑1 (MQ=255)                                 aCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCg                                           <  1:17976/81‑1 (MQ=255)                                  ccGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGt                                          <  2:17978/81‑1 (MQ=255)                                  ccGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGt                                          <  1:17977/81‑1 (MQ=255)                                  ccGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGt                                          >  1:18058/1‑81 (MQ=255)                                    gCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTcc                                        <  1:17979/81‑1 (MQ=255)                                     ccAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCg                                       <  1:17980/81‑1 (MQ=255)                                      cAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCgg                                      >  2:18056/1‑81 (MQ=255)                                        gTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGt                                    >  1:18022/1‑81 (MQ=255)                                         tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTTGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                   >  1:18046/1‑81 (MQ=255)                                         tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                   <  1:17982/81‑1 (MQ=255)                                         tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                   <  2:17983/81‑1 (MQ=255)                                         tAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                   <  2:17981/81‑1 (MQ=255)                                              gTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGTCTATGCAGACGATCGTCCGGGTCgcgc                               >  1:18030/1‑80 (MQ=255)                                              gTGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGcc                              <  1:17984/81‑1 (MQ=255)                                               tGGCAGCAACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCgcgc                               >  1:18048/1‑79 (MQ=255)                                                 gcagcaACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAg                            <  2:17985/80‑1 (MQ=255)                                                    gcaACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCtt                        <  1:17986/81‑1 (MQ=255)                                                     caACAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTg                       >  1:18036/1‑81 (MQ=255)                                                      aaCAGCAGCAGCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTg                       >  1:18034/1‑80 (MQ=255)                                                        cagcagcagCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcag                    >  2:18020/1‑81 (MQ=255)                                                        cagcagcagCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcag                    <  1:17989/81‑1 (MQ=255)                                                        cagcagcagCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcag                    <  1:17987/81‑1 (MQ=255)                                                        cagcagcagCGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcag                    <  2:17988/81‑1 (MQ=255)                                                         agcagcagcGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagc                   <  2:17990/81‑1 (MQ=255)                                                         agcagcagcGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagc                   <  2:17991/81‑1 (MQ=255)                                                              cagcGCCAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgt              <  1:17992/81‑1 (MQ=255)                                                                   ccAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGc         >  1:18069/1‑81 (MQ=255)                                                                    cAGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGcc        <  1:17993/81‑1 (MQ=255)                                                                     aGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCg       <  2:17994/81‑1 (MQ=255)                                                                     aGCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCg       >  2:18042/1‑81 (MQ=255)                                                                      gCACAATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCgg      >  2:18037/1‑81 (MQ=255)                                                                        acaATTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgc    >  2:18039/1‑81 (MQ=255)                                                                           aTTGACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgcgc  >  2:18045/1‑80 (MQ=255)                                                                              |                                                                             CCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGC  >  NC_000913/73564‑73717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑