Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,220	C→T	D320N (GAT→AAT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,220	0	C	T	100.0%	121.6 / NA	34	D320N (GAT→AAT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/12);  total (22/12)

ACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATG  >  NC_000913/698144‑698295                                                                             |                                                                            aCCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATaa                                                                         <  2:150055/81‑1 (MQ=255)    aTTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGtt                                                                      <  2:150056/81‑1 (MQ=255)          aTGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGAt                                                                 <  1:150057/80‑1 (MQ=255)             cccATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTgg                                                             >  1:150083/1‑81 (MQ=255)               cATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGt                                                            >  2:150090/1‑80 (MQ=255)                 tCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTaaa                                                         >  2:150069/1‑81 (MQ=255)                   gCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAAt                                                        >  2:150121/1‑80 (MQ=255)                     cTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCAc                                                     >  1:150099/1‑81 (MQ=255)                      ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACg                                                    >  2:150093/1‑81 (MQ=255)                      ttGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACg                                                    >  2:150096/1‑81 (MQ=255)                        gATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGt                                                   <  2:150058/80‑1 (MQ=255)                                  gACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCa                                        >  1:150086/1‑81 (MQ=255)                                  aaCATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCa                                        >  2:150106/2‑81 (MQ=255)                                        aaaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGa                                  >  2:150108/1‑81 (MQ=255)                                         aaTACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGAc                                 <  1:150059/81‑1 (MQ=255)                                           tACATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCt                               >  1:150100/1‑81 (MQ=255)                                            aCATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCtt                              >  2:150091/1‑81 (MQ=255)                                             cATCGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTc                             >  1:150125/1‑81 (MQ=255)                                                cGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTg                          >  1:150103/1‑81 (MQ=255)                                                cGGTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTg                          >  2:150112/1‑81 (MQ=255)                                                 ggTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTg                          >  1:150097/1‑80 (MQ=255)                                                 ggTGTTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGt                         <  2:150060/81‑1 (MQ=255)                                                  gtgtTGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTa                        <  1:150061/81‑1 (MQ=255)                                                     ttGAAGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACag                     >  1:150102/1‑81 (MQ=255)                                                        aaGCGCGAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACagtga                  <  2:150062/81‑1 (MQ=255)                                                            gcgAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACagtgaagtg              <  1:150063/81‑1 (MQ=255)                                                            gcgAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACagtgaagt               >  2:150107/1‑80 (MQ=255)                                                              gAATAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAgtgaagtgaa            >  2:150104/1‑81 (MQ=255)                                                                aTAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAAtt          <  2:150064/81‑1 (MQ=255)                                                                 tAGTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAAttt         <  2:150065/81‑1 (MQ=255)                                                                   gTGGTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTc        <  1:150066/80‑1 (MQ=255)                                                                     ggTCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGt      >  2:150098/1‑80 (MQ=255)                                                                       tCACATTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGAt   >  2:150129/1‑81 (MQ=255)                                                                        cacaTTATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATg  >  1:150127/1‑81 (MQ=255)                                                                             |                                                                            ACCATTTTAATGCCCATCGCCTTGATCTTACGCGACATTAAATACATCGGTGTTGAAGCGCGAATAGTGGTCACATCATAAGTTTCGATGTGGTAAATCACGTCGCGGATGGCATCCAGACCTTCCTGTACAGTGAAGTGAATTTCGTGATG  >  NC_000913/698144‑698295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑