Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,541	G→T	R213S (CGT→AGT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,541	0	G	T	100.0%	119.0 / NA	34	R213S (CGT→AGT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/20);  total (14/20)

ACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTT  >  NC_000913/698467‑698618                                                                           |                                                                              accaccAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTc                                                                         <  2:150125/81‑1 (MQ=255)  ccaccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCg                                                                        >  2:150164/1‑81 (MQ=255)  ccaccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCg                                                                        >  2:150154/1‑81 (MQ=255)      caGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGtttt                                                                    <  2:217505/81‑1 (MQ=255)         aaaGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGt                                                                 >  1:150179/1‑81 (MQ=255)          aaGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTc                                                                <  1:150126/81‑1 (MQ=255)          aaGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTc                                                                <  2:150127/81‑1 (MQ=255)           agcagcACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCg                                                               <  1:150128/81‑1 (MQ=255)           agcagcACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCg                                                               >  1:150175/1‑81 (MQ=255)              agcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTc                                                            <  1:150129/81‑1 (MQ=255)                   acCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGtt                                                       <  2:150130/81‑1 (MQ=255)                   acCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGt                                                        >  1:150158/1‑80 (MQ=255)                        aaGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCtt                                                  >  2:150162/1‑81 (MQ=255)                         aGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCttt                                                 <  1:150131/81‑1 (MQ=255)                           gCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCa                                               <  1:150132/81‑1 (MQ=255)                               atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCg                                            >  2:150176/1‑80 (MQ=255)                               atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGc                                           <  2:150133/81‑1 (MQ=255)                                      atcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAg                                    >  2:150181/1‑81 (MQ=255)                                          gaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCg                                 >  1:150170/1‑80 (MQ=255)                                              gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCa                            <  2:150134/81‑1 (MQ=255)                                              gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCa                            <  1:150135/81‑1 (MQ=255)                                                  ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGt                          <  2:150136/79‑1 (MQ=255)                                                  ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGt                          <  1:150138/79‑1 (MQ=255)                                                  ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTc                         <  2:150139/80‑1 (MQ=255)                                                  ttAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTc                         <  1:150137/80‑1 (MQ=255)                                                   tAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcg                        <  2:150140/80‑1 (MQ=255)                                                   tAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcg                        <  2:150141/80‑1 (MQ=255)                                                    aaCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgc                       >  2:150180/1‑80 (MQ=255)                                                    aaCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgc                       <  1:150142/80‑1 (MQ=255)                                                     aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTcgcg                      <  2:150143/80‑1 (MQ=255)                                                      cTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCAGCGTAGTCGAACCAGTCGCgat                    >  2:150169/1‑81 (MQ=255)                                                                cAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAg           >  2:150173/1‑80 (MQ=255)                                                                        gACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt  >  2:150166/1‑81 (MQ=255)                                                                        gACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAttt  >  1:150185/1‑81 (MQ=255)                                                                           |                                                                              ACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGATTT  >  NC_000913/698467‑698618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑