Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	750,915	C→T	D419N (GAT→AAT)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	750,915	0	C	T	100.0%	81.1 / NA	23	D419N (GAT→AAT)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

ATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGC  >  NC_000913/750839‑750990                                                                             |                                                                            aTCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTaaaa                                                                         <  1:159763/81‑1 (MQ=255) aTCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTaaaa                                                                         <  1:159762/81‑1 (MQ=255)  tCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAAt                                                                        <  1:159764/81‑1 (MQ=255)  tCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAAt                                                                        >  2:159797/1‑81 (MQ=255)          ttGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTg                                                                >  1:159811/1‑81 (MQ=255)             gtggCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGcc                                                             >  1:159799/1‑81 (MQ=255)               ggCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATTGCTACTGGcc                                                             >  1:159818/1‑79 (MQ=255)                      aCTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACa                                                    >  2:159812/1‑81 (MQ=255)                              gCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAgg                                             <  1:159765/80‑1 (MQ=255)                               cTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGa                                            >  1:159808/1‑80 (MQ=255)                                  tAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGt                                        <  2:159766/81‑1 (MQ=255)                                    aTTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTgc                                      <  1:159768/81‑1 (MQ=255)                                    aTTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTg                                       <  2:159767/80‑1 (MQ=255)                                       aaaaCGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTgcgc                                    >  2:159810/1‑80 (MQ=255)                                          aCGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAg                                <  1:159769/81‑1 (MQ=255)                                            ggTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGcc                              <  1:159770/81‑1 (MQ=255)                                            ggTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGc                               >  2:159824/1‑80 (MQ=255)                                                    aaGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCat                       <  2:159771/80‑1 (MQ=255)                                                    aaGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCa                        <  1:159772/79‑1 (MQ=255)                                                     aGCCCCGCTCGTCCTGCTGAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCatat                     >  2:159773/1‑81 (MQ=255)                                                                     ctgAGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATAccc       <  2:159774/79‑1 (MQ=255)                                                                        aGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCAtt    <  2:159775/79‑1 (MQ=255)                                                                        aGTATTAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGc  <  1:159776/81‑1 (MQ=255)                                                                             |                                                                            ATCGTGCTGTTGGTGGCATCCACTTGTTTGCTGTAATTAAAACGGTAGCTTAAGCCCCGCTCGTCCTGCTGAGTATCAAAATGGCTACTGGCCCAGGTGACATCAAAGGACAGTGCGCCAAGCCACAGCATATTTTGCCCGATACCCATTGC  >  NC_000913/750839‑750990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑