Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	785,304	T→C	K5R (AAA→AGA)	ybgS ←	PF13985 family protein YbgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	785,304	0	T	C	100.0%	73.7 / NA	22	K5R (AAA→AGA)	ybgS	PF13985 family protein YbgS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/13);  total (9/13)

GTTTGCGCTCCGCTATCGGCAGCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTTTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTGCAGGTG  >  NC_000913/785226‑785378                                                                               |                                                                           gTTTGCGCTCCGCTATCGGCAGCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGtctt                                                                          <  1:166222/81‑1 (MQ=255)    tGCGCTCCGCTATCGGCAGCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGt                                                                        >  2:166280/1‑80 (MQ=255)                tCGGCAGCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAg                                                             <  1:166224/79‑1 (MQ=255)                      gCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTcc                                                     >  2:166274/1‑81 (MQ=255)                         aGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGt                                                  >  2:166284/1‑81 (MQ=255)                             cgacACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATg                                              <  2:166226/78‑1 (MQ=255)                             cGGCACCGCTGGCAAGGCTTAGAGTGGCAGTTAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATg                                              <  1:166225/81‑1 (MQ=255)                                 aCCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCg                                          >  1:166269/1‑81 (MQ=255)                                     cTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTAt                                      <  1:166227/81‑1 (MQ=255)                                       ggCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGt                                    >  2:166275/1‑81 (MQ=255)                                            ggCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGa                                >  2:166271/1‑80 (MQ=255)                                              cTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATaa                             >  1:166267/1‑81 (MQ=255)                                                  gagTGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACct                          <  1:166228/80‑1 (MQ=255)                                                     tGGCAGTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTc                      <  1:166229/81‑1 (MQ=255)                                                          gTCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACa                 <  2:166230/81‑1 (MQ=255)                                                           tCAGAAATAATGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAg                <  1:166231/81‑1 (MQ=255)                                                                aataatGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCAt           >  1:166285/1‑81 (MQ=255)                                                                 ataatGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCAtt          <  1:166232/81‑1 (MQ=255)                                                                 ataatGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCAtt          >  1:166282/1‑81 (MQ=255)                                                                    atGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTg        <  2:166234/80‑1 (MQ=255)                                                                    atGTGGCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTGc       <  2:166233/81‑1 (MQ=255)                                                                         gCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTGCAGGTg  <  1:166235/81‑1 (MQ=255)                                                                         gCCAGTCTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTGCAGGTg  <  1:166236/81‑1 (MQ=255)                                                                               |                                                                           GTTTGCGCTCCGCTATCGGCAGCCAGTGCGGCACCGCTGGCAAGGCTTAGAGTGGCAGTCAGAAATAATGTGGCCAGTTTTGTCATTTTCATAGGATGCTCCTGTTATGGTCGTTATGTCGGATAACCTCTTCCAACAGTGCATTTGCAGGTG  >  NC_000913/785226‑785378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑