Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,736	T→C	E232E (GAA→GAG)	ybhA ←	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,736	0	T	C	100.0%	88.2 / NA	26	E232E (GAA→GAG)	ybhA	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/18);  total (8/18)

TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797659‑797813                                                                              |                                                                              ttATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTcc                                                                            <  1:168730/81‑1 (MQ=255) ttATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTc                                                                             >  1:168768/1‑80 (MQ=255)  tATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTc                                                                             >  2:168780/1‑79 (MQ=255)    tCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAg                                                                          <  1:168732/80‑1 (MQ=255)    tCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGc                                                                         <  1:168731/81‑1 (MQ=255)      accaaccaCAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCAt                                                                       <  2:168733/81‑1 (MQ=255)             acaATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGata                                                                >  2:168763/1‑81 (MQ=255)             acaATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGat                                                                 <  1:168734/80‑1 (MQ=255)              caATGTTGGCGCGCGCTTTTACCGCGTCATCAGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGatat                                                               <  2:168735/81‑1 (MQ=255)               aaTGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTTCCAGCGGCCTCCAGCATACTGATATc                                                              <  2:168736/81‑1 (MQ=255)                aTGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCa                                                             <  1:168737/81‑1 (MQ=255)                   ttGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCAtt                                                           <  2:168738/80‑1 (MQ=255)                        gcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGtt                                                     <  2:168739/81‑1 (MQ=255)                           cgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATc                                                  >  2:168782/1‑81 (MQ=255)                            gcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCg                                                 >  1:168776/1‑81 (MQ=255)                            gcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCg                                                 <  2:168740/81‑1 (MQ=255)                                ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGcc                                               <  1:168741/79‑1 (MQ=255)                                ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGCACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCg                                              <  2:168742/80‑1 (MQ=255)                                 tACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGaa                                            >  2:168794/1‑81 (MQ=255)                                     gcgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGcc                                        <  1:168743/81‑1 (MQ=255)                                       gTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCac                                      <  2:168744/81‑1 (MQ=255)                                                  tGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCa                           >  2:168781/1‑81 (MQ=255)                                                    cccATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCAt                          <  2:168745/80‑1 (MQ=255)                                                        tCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGAcc                     <  1:168746/81‑1 (MQ=255)                                                          gCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCaa                   <  2:168747/81‑1 (MQ=255)                                                                           ggCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCa  >  2:168799/1‑81 (MQ=255)                                                                              |                                                                              TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797659‑797813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑