Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,565	G→A	P392L (CCG→CTG)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,565	0	G	A	100.0%	102.3 / NA	29	P392L (CCG→CTG)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

ATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAAC  >  NC_000913/822489‑822642                                                                             |                                                                              aTTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATc                                                                           <  2:173373/81‑1 (MQ=255)         atcaGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTgcg                                                                   >  2:173459/1‑81 (MQ=255)               gTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGAc                                                             <  1:173374/81‑1 (MQ=255)                  ttACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGt                                                           <  2:173375/80‑1 (MQ=255)                   tACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTCGTGCGGGCAGATCAACCTGCGCCAGACGTgg                                                         >  2:173403/1‑81 (MQ=255)                    aCCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTgg                                                         <  1:173376/80‑1 (MQ=255)                    aCCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGc                                                        >  2:173415/1‑81 (MQ=255)                    aCCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGc                                                        >  2:173404/1‑81 (MQ=255)                        ccGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCgtgt                                                    <  2:173377/81‑1 (MQ=255)                         cGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCgtgtg                                                   >  1:173409/1‑81 (MQ=255)                         cGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCgtgtg                                                   <  1:173378/81‑1 (MQ=255)                              tttCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCg                                              >  1:173417/1‑81 (MQ=255)                                   gTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAagc                                         >  1:173422/1‑81 (MQ=255)                                   gTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAagc                                         >  2:173416/1‑81 (MQ=255)                                   gTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAagc                                         <  1:173379/81‑1 (MQ=255)                                    tttCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAagcc                                        <  2:173380/81‑1 (MQ=255)                                             gATCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCa                               <  1:173381/81‑1 (MQ=255)                                              aTCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAg                              <  2:173382/81‑1 (MQ=255)                                               tCCTGCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAgc                             <  2:173383/81‑1 (MQ=255)                                                   gCGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCg                         >  1:173430/1‑81 (MQ=255)                                                    cGTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGcc                          <  2:173384/79‑1 (MQ=255)                                                     gTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCggg                       <  1:173385/81‑1 (MQ=255)                                                     gTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCgg                        >  2:173414/1‑80 (MQ=255)                                                     gTTTCCATTGTCGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCgg                        >  1:173424/1‑80 (MQ=255)                                                                cGGTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTa             >  2:173425/1‑80 (MQ=255)                                                                 ggTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTaa            >  2:173412/1‑80 (MQ=255)                                                                 ggTTGTGCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTaa            >  2:173426/1‑80 (MQ=255)                                                                     gtgCGGGCAGATCAACCT‑‑GCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTg      >  2:173433/1‑80 (MQ=255)                                                                      tgCGGGCAGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTg      >  1:173435/1‑81 (MQ=255)                                                                          ggcagATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAAc  >  2:173423/1‑81 (MQ=255)                                                                             |                                                                              ATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAAC  >  NC_000913/822489‑822642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑