Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	850,566	C→G	G39G (GGC→GGG)	ompX →	outer membrane protein X

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	850,566	0	C	G	100.0%	51.2 / NA	16	G39G (GGC→GGG)	ompX	outer membrane protein X
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/5);  total (11/5)

CCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTT  >  NC_000913/850499‑850642                                                                    |                                                                             ccGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACaaaa                                                                 <  1:178696/81‑1 (MQ=255)         aCTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGt                                                         >  1:178729/1‑81 (MQ=255)            tCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGttt                                                       >  1:178720/1‑80 (MQ=255)                      cGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAAt                                            <  1:178697/81‑1 (MQ=255)                      cGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTCCAACCTGaaa                                             >  2:178723/1‑80 (MQ=255)                                 gTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATg                                   >  1:178731/1‑79 (MQ=255)                                    aCTGGCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATgaag                                >  2:178728/1‑79 (MQ=255)                                        gCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAagaaga                            <  2:178698/79‑1 (MQ=255)                                        gCGGTTACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGac                           <  1:178699/80‑1 (MQ=255)                                             tACGCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGc                     >  2:178736/1‑81 (MQ=255)                                                gCACAGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCg                  >  1:178726/1‑81 (MQ=255)                                                  acaGAGCGACGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCt                >  1:178730/1‑81 (MQ=255)                                                  acaGAGCGACACTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCt                <  1:178700/81‑1 (MQ=255)                                                           cGCTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGAt       >  2:178727/1‑81 (MQ=255)                                                             cTCAGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCg     >  2:178740/1‑81 (MQ=255)                                                                aGGGGCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGtt  >  2:178732/1‑81 (MQ=255)                                                                    |                                                                             CCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGGTTTCAACCTGAAATACCGCTATGAAGAAGACAACAGCCCGCTGGGTGTGATCGGTT  >  NC_000913/850499‑850642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑