Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	857,021	C→A	T353T (ACC→ACA)	ybiT →	ABC transporter ATP‑binding protein YbiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	857,021	0	C	A	100.0%	95.5 / NA	27	T353T (ACC→ACA)	ybiT	ABC transporter ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/15);  total (12/15)

GTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTC  >  NC_000913/856946‑857098                                                                            |                                                                              gTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACgg                                                                          <  1:179784/81‑1 (MQ=255)       tAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCgg                                                                    <  1:179785/81‑1 (MQ=255)         aCGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGt                                                                   >  2:179840/1‑80 (MQ=255)            gTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAAt                                                               <  2:179786/81‑1 (MQ=255)             tCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAAt                                                               >  1:179813/1‑80 (MQ=255)             tCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAAt                                                               >  1:179812/1‑80 (MQ=255)                       aaaaaTCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACgctgct                                                     <  1:179787/80‑1 (MQ=255)                        aaaaTCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGa                                                   <  1:179788/81‑1 (MQ=255)                         aaaTCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGaa                                                  <  1:179789/81‑1 (MQ=255)                                aaCCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAAc                                               <  1:179791/77‑1 (MQ=255)                                aaCCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCtg                                           <  2:179790/81‑1 (MQ=255)                                 aCCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCtgg                                          >  1:179818/1‑81 (MQ=255)                                     gctgctgGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTggg                                      >  1:179820/1‑81 (MQ=255)                                     gctgctgGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTggg                                      >  1:179828/1‑81 (MQ=255)                                        gctgGAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGa                                   >  1:179834/1‑81 (MQ=255)                                            gAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTg                               <  2:179792/81‑1 (MQ=255)                                            gAAGTGGGTGAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTg                               >  2:179824/1‑81 (MQ=255)                                                     gAAAAACTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGa                       >  1:179819/1‑80 (MQ=255)                                                      aaaaaCTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGAc                      <  1:179793/80‑1 (MQ=255)                                                         aaCTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCg                  >  2:179829/1‑81 (MQ=255)                                                           cTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGc                <  2:179794/81‑1 (MQ=255)                                                           cTGGCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCCGGTGGGCGATCTGCAACCGGACAGCGGc                <  1:179795/81‑1 (MQ=255)                                                              gCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCa               <  2:179797/79‑1 (MQ=255)                                                              gCGGTACTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCAc              <  1:179796/80‑1 (MQ=255)                                                                   aCTGGGTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTaa         >  1:179844/1‑80 (MQ=255)                                                                      gggTACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATg     >  2:179831/1‑81 (MQ=255)                                                                         tACAAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTc  <  2:179798/81‑1 (MQ=255)                                                                            |                                                                              GTTTGATAACGGTCCGCTGTTTAAAAATCTCAACCTGCTGCTGGAAGTGGGTGAAAAACTGGCGGTACTGGGTACCAACGGCGTCGGTAAATCAACGCTGCTGAAAACGCTGGTGGGCGATCTGCAACCGGACAGCGGCACCGTAAAATGGTC  >  NC_000913/856946‑857098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑