| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 868,871 | G→C | G451A (GGC→GCC) | gsiA → | glutathione ABC transporter ATP binding subunit GsiA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 868,871 | 0 | G | C | 96.4% | 86.6 / ‑5.7 | 28 | G451A (GGC→GCC) | gsiA | glutathione ABC transporter ATP binding subunit GsiA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (15/12); minor base A (0/1); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCATTGCTC > NC_000913/868794‑868945 | cGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCt > 1:182208/1‑81 (MQ=255) gtgtACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGaa > 1:182213/1‑80 (MQ=255) tACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATg < 2:182165/81‑1 (MQ=255) cacGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGcc < 1:182166/81‑1 (MQ=255) acGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCt > 1:182216/1‑81 (MQ=255) ggTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTgg > 2:182197/1‑81 (MQ=255) gTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGc > 1:182209/1‑81 (MQ=255) ttGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTAc > 2:182206/1‑81 (MQ=255) gCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTAcc < 2:182167/80‑1 (MQ=255) gCCAGGTAAAGACGCGGCTGAACGCGTTGCGTGGTTGCTGGAGCGCGTGGACCTGTTACCTGAACATGCCTGGCGTTAcc < 2:182168/80‑1 (MQ=255) cAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGc < 1:182169/81‑1 (MQ=255) gTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATg < 2:182170/81‑1 (MQ=255) aaaGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAg > 2:182211/1‑81 (MQ=255) aaGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGt > 2:182210/1‑81 (MQ=255) gcgGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTcc > 2:182217/1‑81 (MQ=255) cgGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCcg < 2:182171/81‑1 (MQ=255) gCACGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGt > 2:182215/1‑81 (MQ=255) aCGCGTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGt > 1:182225/1‑79 (MQ=255) gTTGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTcagcgc > 2:182207/1‑81 (MQ=255) ttGCGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTcagcgcc < 1:182172/81‑1 (MQ=255) cGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTcagcgccag < 2:182174/80‑1 (MQ=255) cGTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTcagcgccag < 1:182173/80‑1 (MQ=255) gTGGTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTcagcgccagcg < 2:182175/81‑1 (MQ=255) gTTGCTGGAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCa < 1:182176/80‑1 (MQ=255) ggAGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCAt > 2:182205/1‑81 (MQ=255) aGCGCGTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCATTg > 2:182214/1‑81 (MQ=255) cgcgTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCATTGCt > 2:182222/1‑81 (MQ=255) gcgTGGCCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCATTGCTc < 1:182177/81‑1 (MQ=255) | CGAACCGCTGCGTGTACACGGTTTATTGCCAGGTAAAGACGCGGCTGCACGCGTTGCGTGGTTGCTGGAGCGCGTGGGCCTGTTACCTGAACATGCCTGGCGTTACCCGCATGAGTTTTCCGGCGGTCAGCGCCAGCGCATCTGCATTGCTC > NC_000913/868794‑868945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |