Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	871,256	C→T	P97L (CCA→CTA)	gsiC →	glutathione ABC transporter membrane subunit GsiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	871,256	0	C	T	100.0%	106.5 / NA	30	P97L (CCA→CTA)	gsiC	glutathione ABC transporter membrane subunit GsiC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

TGCTGTGCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCCAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTATCGCCGCCGTC  >  NC_000913/871179‑871332                                                                              |                                                                             tGCTGTGCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAAc                                                                           <  2:182565/81‑1 (MQ=255)  gCTGTGCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACg                                                                          >  2:182605/1‑81 (MQ=255)   cTGTGCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACg                                                                          <  1:182566/80‑1 (MQ=255)       gCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCtctg                                                                     >  2:182607/1‑81 (MQ=255)         aGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGc                                                                   <  2:182567/81‑1 (MQ=255)         aGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGc                                                                   >  2:182616/1‑81 (MQ=255)              gATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGAc                                                               >  2:182614/1‑80 (MQ=255)                ttttGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCAt                                                            <  1:182568/81‑1 (MQ=255)                  ttGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATaa                                                          >  1:182603/1‑81 (MQ=255)                    ggCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAAc                                                         >  2:182611/1‑80 (MQ=255)                          tCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTa                                                    <  2:182569/79‑1 (MQ=255)                            gATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATgg                                                 <  1:182570/80‑1 (MQ=255)                            gATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGt                                                >  1:182618/1‑81 (MQ=255)                              tGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCt                                              >  2:182622/1‑81 (MQ=255)                               ggTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTg                                             >  2:182615/1‑81 (MQ=255)                                     gcgTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCgg                                        <  1:182571/80‑1 (MQ=255)                                      cgtcgtCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGtt                                      <  2:182572/81‑1 (MQ=255)                                       gtcgtcCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTta                                     >  1:182620/1‑81 (MQ=255)                                       gtcgtcCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTta                                     >  1:182632/1‑81 (MQ=255)                                                 tGCCGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTAt                           >  1:182624/1‑81 (MQ=255)                                                   ccGATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATgg                         <  2:182573/81‑1 (MQ=255)                                                     gATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATggcg                       >  1:182630/1‑81 (MQ=255)                                                     gATGAGATTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATggcg                       >  1:182627/1‑81 (MQ=255)                                                          gaTTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGcggcgg                   >  2:182638/1‑80 (MQ=255)                                                           aTTGCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGTCGGCggg                  >  1:182621/1‑80 (MQ=255)                                                              gCCAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAAt               <  1:182574/80‑1 (MQ=255)                                                                cAGCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTAt            >  1:182626/1‑81 (MQ=255)                                                                  gCCGCTTTATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTATcg          <  1:182575/81‑1 (MQ=255)                                                                         tATGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTATCgccgcc     <  1:182576/79‑1 (MQ=255)                                                                           tGCTAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTATCGCCGCCGTc  <  1:182577/80‑1 (MQ=255)                                                                              |                                                                             TGCTGTGCAGGGGGATTTTGGCCTGTCGATGGTGTCGCGTCGTCCGGTTGCCGATGAGATTGCCAGCCGCTTTATGCCAACGCTGTGGCTGACCATAACCAGTATGGTCTGGGCGGTTATATTTGGTATGGCGGCGGGAATTATCGCCGCCGTC  >  NC_000913/871179‑871332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑