Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	878,719	G→A	intergenic (+88/‑23)	bssR → / → yliI	regulator of biofilm formation/aldose sugar dehydrogenase YliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	878,719	0	G	A	100.0%	95.9 / NA	27	intergenic (+88/‑23)	bssR/yliI	regulator of biofilm formation/aldose sugar dehydrogenase YliI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/10);  total (17/10)

AAAGCGTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTC  >  NC_000913/878646‑878795                                                                          |                                                                             aaaGCGTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGt                                                                       <  1:183732/81‑1 (MQ=255)     cGTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAAt                                                                   >  1:183778/1‑81 (MQ=255)      gTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATc                                                                  >  2:183760/1‑81 (MQ=255)          cccccTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCg                                                                >  1:183774/1‑79 (MQ=255)                tCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGt                                                         >  2:183767/1‑80 (MQ=255)                tCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGt                                                         >  2:183768/1‑80 (MQ=255)                 cATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGtt                                                        <  2:183733/80‑1 (MQ=255)                 cATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTa                                                       >  2:183759/1‑81 (MQ=255)                     tttAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCa                                                   >  1:183772/1‑81 (MQ=255)                       tAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATc                                                 >  2:183770/1‑81 (MQ=255)                         aGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGa                                               >  1:183769/1‑81 (MQ=255)                         aGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGa                                               >  2:183762/1‑81 (MQ=255)                              ttGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAAt                                           <  2:183734/80‑1 (MQ=255)                                gTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCt                                        >  2:183742/1‑81 (MQ=255)                                 tAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCtt                                       >  1:183779/1‑81 (MQ=255)                                 tAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCtt                                       >  2:183781/1‑81 (MQ=255)                                   aaaaTGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCttt                                      <  1:183735/80‑1 (MQ=255)                                     aaTGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTc                                   <  2:183737/81‑1 (MQ=255)                                     aaTGAATTTGTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTc                                   <  2:183736/81‑1 (MQ=255)                                              gTTATCTCCTCCACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGccc                          >  2:183766/1‑81 (MQ=255)                                                      ctccACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTa                      <  2:183739/77‑1 (MQ=255)                                                      ctccACTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTAtt                    <  2:183738/79‑1 (MQ=255)                                                          aCTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGtctt              >  1:183771/1‑81 (MQ=255)                                                           cTGACTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGtcttt             <  2:183740/81‑1 (MQ=255)                                                               cTACGCTTTAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTttcttc         >  1:183765/1‑81 (MQ=255)                                                                      ttAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGctctc  >  1:183776/1‑81 (MQ=255)                                                                       tAAACCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGctct   <  1:183741/79‑1 (MQ=255)                                                                          |                                                                             AAAGCGTCTCCCCCTTCATGTTTAAGTTTTTGTAAAAATGAATTTGTTATCTCCTCCACTGACTACGCTTTAAGCCAGAGTCAATCCGGAGGCGTTATGCATCGACAATCCTTTTTCCTTGTGCCCCTTATTTGTCTTTCTTCCGCTCTC  >  NC_000913/878646‑878795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑