| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 885,221 | T→C | K4E (AAA→GAA) | ybjH ← | uncharacterized protein YbjH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 885,221 | 0 | T | C | 97.1% | 115.9 / ‑5.6 | 35 | K4E (AAA→GAA) | ybjH | uncharacterized protein YbjH |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (7/27); total (8/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACATCGACGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTG > NC_000913/885145‑885292 | acaTCGACGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATa < 1:184817/81‑1 (MQ=255)acaTCGACGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATa < 2:184816/81‑1 (MQ=255) cGACGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCa > 2:184869/1‑81 (MQ=255) aCGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCAtt < 2:184818/81‑1 (MQ=255) ggTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTc < 1:184819/81‑1 (MQ=255) ggTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTc > 1:184858/1‑81 (MQ=255) tGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGc < 1:184820/81‑1 (MQ=255) gACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTACAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCTcc > 2:184821/1‑81 (MQ=255) cacTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTg < 2:184823/81‑1 (MQ=255) cacTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTg < 2:184822/81‑1 (MQ=255) gcgcCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAaata < 2:184824/80‑1 (MQ=255) cgcCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAaataat < 1:184825/81‑1 (MQ=255) cATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAATAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCa < 1:184826/81‑1 (MQ=255) aTCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAg < 1:184827/81‑1 (MQ=255) tCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGc > 2:184873/1‑81 (MQ=255) tCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGc < 2:184829/81‑1 (MQ=255) tCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGc < 2:184828/81‑1 (MQ=255) tCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGc > 1:184880/1‑81 (MQ=255) gCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCgg > 2:184879/1‑81 (MQ=255) acacCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCCCCTGAAATAATGCAGCGGGtt < 1:184830/81‑1 (MQ=255) acCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTaa > 1:184876/1‑81 (MQ=255) ccAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAAt < 1:184831/81‑1 (MQ=255) aGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAAttt < 2:184832/81‑1 (MQ=255) tAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTcc < 2:184833/81‑1 (MQ=255) gCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGa < 1:184834/81‑1 (MQ=255) ttAAAAGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTAt < 1:184835/81‑1 (MQ=255) aaaGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGc < 1:184836/80‑1 (MQ=255) aGCGCGCCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGc < 2:184837/81‑1 (MQ=255) gcgcgcCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGcc < 2:184838/81‑1 (MQ=255) gcgcgcCCAACAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGcc < 2:184839/81‑1 (MQ=255) aaCAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCttcatt > 2:184871/1‑79 (MQ=255) aCAGTAGACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAg < 1:184840/81‑1 (MQ=255) gACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCAtt < 1:184843/80‑1 (MQ=255) gACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCAtt < 2:184842/80‑1 (MQ=255) gACAATTTTCCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATtg < 1:184841/81‑1 (MQ=255) | ACATCGACGGTGACACTTTGCGCCATCGCCACACCAGTAAAGCCCATTAAAAGCGCGCCCAACAGTAGACAATTTTTCATAATCATTTCGCCTGAAATAATGCAGCGGGTTAATTTCCGCGAATTATGCAGCCTTCATTCAGGCATTG > NC_000913/885145‑885292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |