Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	886,780	A→G	I187T (ATT→ACT)	ybjJ ←	inner membrane protein YbjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	886,780	0	A	G	100.0%	110.5 / NA	32	I187T (ATT→ACT)	ybjJ	inner membrane protein YbjJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

CGATAAAAAGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAATGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGCCA  >  NC_000913/886702‑886848                                                                               |                                                                     cgATAAAAAGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTg                                                                    <  2:185098/81‑1 (MQ=255)  gATAAAAAGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTgg                                                                   <  1:185099/81‑1 (MQ=255)  gATAAAAAGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTgg                                                                   >  1:185139/1‑81 (MQ=255)      aaaaGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGat                                                               <  1:185100/81‑1 (MQ=255)      aaaaGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGat                                                               <  2:185101/81‑1 (MQ=255)       aaaGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGata                                                              <  1:185102/81‑1 (MQ=255)       aaaGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGata                                                              >  2:185151/1‑81 (MQ=255)        aaGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGata                                                              >  1:185153/1‑80 (MQ=255)         aGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGatat                                                             >  1:185140/1‑80 (MQ=255)          ggTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGAtata                                                            >  1:185149/1‑80 (MQ=255)             aCGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAAt                                                         >  2:185228/1‑80 (MQ=255)              cGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAg                                                       >  2:185150/1‑81 (MQ=255)                    tttCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGc                                                   <  1:185103/79‑1 (MQ=255)                     ttCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGa                                                 >  1:185145/1‑80 (MQ=255)                                  gTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGcgcc                                   <  2:185104/81‑1 (MQ=255)                                       aTCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCaa                              >  1:185196/1‑81 (MQ=255)                                        tCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCaa                              >  1:185163/1‑80 (MQ=255)                                          ggCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATaa                           <  1:185105/81‑1 (MQ=255)                                             agcaTTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAAt                        <  2:185106/81‑1 (MQ=255)                                                aTTTTTGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAAtgtg                     >  2:185131/1‑81 (MQ=255)                                                     tGCCCGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCAcc                 <  2:185107/80‑1 (MQ=255)                                                        ccGTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTg             <  1:185108/81‑1 (MQ=255)                                                          gTACCGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGACAATAAAATGTGCACCGTTGcc           <  1:185109/81‑1 (MQ=255)                                                             ccGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGa        <  1:185110/81‑1 (MQ=255)                                                              cGTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGaa       >  1:185143/1‑81 (MQ=255)                                                               gTCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAAc      >  1:185147/1‑81 (MQ=255)                                                                tCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACg     >  2:185148/1‑81 (MQ=255)                                                                tCAGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACg     >  2:185157/1‑81 (MQ=255)                                                                  aGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGcc   <  2:185111/81‑1 (MQ=255)                                                                  aGGGATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGc    >  1:185154/1‑80 (MQ=255)                                                                   gggATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGCCa  <  2:185112/81‑1 (MQ=255)                                                                    ggATTGCCTGAGTGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGCCa  >  1:185158/1‑80 (MQ=255)                                                                               |                                                                     CGATAAAAAGGTACGCCTTTTTCGCCATGCTGGGTGCCATCGGCAGCATTTTTGCCCGTACCGTCAGGGATTGCCTGAATGGCGATATAAATAGGTGCGATACCTACCAGCGCCGCCAATAAAATGTGCACCGTTGCCGGAACGCCA  >  NC_000913/886702‑886848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑