Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	894,965	C→A	intergenic (+69/‑26)	potF → / → potG	putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	894,965	0	C	A	100.0%	130.2 / NA	36	intergenic (+69/‑26)	potF/potG	putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/18);  total (18/18)

GGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAC  >  NC_000913/894888‑895040                                                                              |                                                                            ggAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGaaca                                                                          <  2:186502/81‑1 (MQ=255)     aataatCCGCAGTCGTAGATGCCGGAGGGGCACACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACAtt                                                                      <  2:186503/81‑1 (MQ=255)      ataatCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACAtt                                                                      >  1:186563/1‑80 (MQ=255)           ccGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGc                                                                >  1:186542/1‑81 (MQ=255)             gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCg                                                              <  2:186505/81‑1 (MQ=255)             gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCg                                                              <  2:186504/81‑1 (MQ=255)             gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCg                                                              <  1:186506/81‑1 (MQ=255)             gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCg                                                              >  2:186565/1‑81 (MQ=255)             gCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCg                                                              >  1:186568/1‑81 (MQ=255)                     aGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGagcag                                                      >  1:186558/1‑81 (MQ=255)                      gATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGagcagc                                                     >  1:186566/1‑81 (MQ=255)                           cGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTg                                                 >  1:186572/1‑80 (MQ=255)                            ggAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGaa                                               >  2:186560/1‑81 (MQ=255)                            ggAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGaa                                               >  1:186556/1‑81 (MQ=255)                             gAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAAt                                              <  2:186507/81‑1 (MQ=255)                               ggggCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGa                                            <  1:186508/81‑1 (MQ=255)                                         acacCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCt                                  <  2:186509/81‑1 (MQ=255)                                          cacCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTc                                 <  2:186510/81‑1 (MQ=255)                                           acCCGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCg                                >  1:186553/1‑81 (MQ=255)                                            cccGCCGGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGc                               <  2:186511/81‑1 (MQ=255)                                             ccgccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGcc                              <  1:186513/81‑1 (MQ=255)                                             ccgccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGcc                              <  1:186512/81‑1 (MQ=255)                                               gccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCg                            >  1:186550/1‑81 (MQ=255)                                               gccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCg                            >  2:186564/1‑81 (MQ=255)                                                ccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGc                           <  2:186514/81‑1 (MQ=255)                                                ccgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGc                           <  2:186515/81‑1 (MQ=255)                                                 cgGCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCg                            >  2:186567/1‑79 (MQ=255)                                                  ggCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                         >  1:186570/1‑81 (MQ=255)                                                  ggCAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAg                         <  1:186516/81‑1 (MQ=255)                                                    cAATTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGc                       <  1:186517/81‑1 (MQ=255)                                                     aaTTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCg                      <  2:186518/81‑1 (MQ=255)                                                      aTTCGCACCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCg                      >  1:186573/1‑80 (MQ=255)                                                            aCCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAccc               >  1:186562/1‑81 (MQ=255)                                                            aCCATTATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAAccc               >  1:186547/1‑81 (MQ=255)                                                                 tATGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAg          >  2:186559/1‑81 (MQ=255)                                                                   tGGTGCGCTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGgc        <  1:186519/81‑1 (MQ=255)                                                                         gcTTGAACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAc  <  1:186520/81‑1 (MQ=255)                                                                              |                                                                            GGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCGCCCGCAGGCGAAAACCCGTAAGGCGCTGAC  >  NC_000913/894888‑895040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑