Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	907,393	T→C	E297G (GAG→GGG)	ybjT ←	putative NAD(P)‑dependent oxidoreductase YbjT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	907,393	0	T	C	100.0%	92.1 / NA	27	E297G (GAG→GGG)	ybjT	putative NAD(P)‑dependent oxidoreductase YbjT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

AACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCTCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCG  >  NC_000913/907316‑907461                                                                              |                                                                     aaCCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCtt                                                                   >  1:188848/1‑81 (MQ=255)  aCCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCtt                                                                   <  1:188805/80‑1 (MQ=255)    cGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTc                                                                 >  2:188853/1‑80 (MQ=255)        cTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGt                                                            <  1:188806/81‑1 (MQ=255)          ccGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGc                                                          >  2:188839/1‑81 (MQ=255)           cGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGc                                                          >  2:188850/1‑80 (MQ=255)           cGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCt                                                         <  1:188807/81‑1 (MQ=255)            ggTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTa                                                        <  2:188808/81‑1 (MQ=255)             gTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTAc                                                       >  1:188845/1‑81 (MQ=255)                         cAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCAt                                           <  1:188809/81‑1 (MQ=255)                             ggCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGa                                        >  2:188863/1‑80 (MQ=255)                               ccTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAg                                     <  2:188810/81‑1 (MQ=255)                               ccTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAg                                     >  1:188846/1‑81 (MQ=255)                                      gtcgtAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAg                              >  2:188857/1‑81 (MQ=255)                                         gtAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCg                           >  2:188858/1‑81 (MQ=255)                                               ccAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTgg                     <  2:188811/81‑1 (MQ=255)                                                cAGTCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTggg                    <  2:188812/81‑1 (MQ=255)                                                   tCGCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATg                 <  2:188813/81‑1 (MQ=255)                                                     gCTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGa                <  2:188814/80‑1 (MQ=255)                                                      cTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGt              >  1:188855/1‑81 (MQ=255)                                                      cTGGAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGt              >  1:188851/1‑81 (MQ=255)                                                        ggAGTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTg             >  2:188854/1‑80 (MQ=255)                                                           gTTGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACg         >  1:188856/1‑81 (MQ=255)                                                            ttGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGt        <  1:188816/81‑1 (MQ=255)                                                            ttGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGt        <  1:188815/81‑1 (MQ=255)                                                             tGACCAGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACg         >  1:188852/1‑79 (MQ=255)                                                                  aGTTTTTCCTCCCCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGcgcg  >  1:188861/1‑81 (MQ=255)                                                                              |                                                                     AACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTAGCCCCAGTCGCTGGAGTTGACCAGTTTTTCCTCCTCTTTCAACGTGCTACGTACCGCGTCATCGAAAGCGATCAGCCGTTGTGGGATGAGTGCACGTAGCGCG  >  NC_000913/907316‑907461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑