Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	918,558	C→A	C188F (TGT→TTT)	ybjX ←	DUF535 domain‑containing protein YbjX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	918,558	0	C	A	100.0%	108.1 / NA	31	C188F (TGT→TTT)	ybjX	DUF535 domain‑containing protein YbjX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/21);  total (10/21)

GATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATC  >  NC_000913/918482‑918634                                                                             |                                                                             gATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTcaaac                                                                            <  1:190870/79‑1 (MQ=255)           tgtgGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGt                                                                 >  1:190922/1‑80 (MQ=255)           tgtgGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTg                                                                <  1:190872/81‑1 (MQ=255)               gAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGAtct                                                            <  1:190873/81‑1 (MQ=255)                 aTTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATctct                                                          <  1:190875/81‑1 (MQ=255)                 aTTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATctct                                                          <  1:190874/81‑1 (MQ=255)                  tttCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTg                                                         <  1:190876/81‑1 (MQ=255)                    tCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGc                                                        >  2:190912/1‑80 (MQ=255)                     cccATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGcc                                                       <  1:190877/80‑1 (MQ=255)                        aTTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCagag                                                   <  1:190878/81‑1 (MQ=255)                         ttttGCGCCTTGCAGTCCGCCAATAAACATCATTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAgg                                                  <  2:190879/81‑1 (MQ=255)                             gcgcCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATa                                              >  2:190921/1‑81 (MQ=255)                              cgcCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATAc                                             <  1:190880/81‑1 (MQ=255)                               gcCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATAcc                                            >  2:190915/1‑81 (MQ=255)                                ccTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCt                                           >  2:190913/1‑81 (MQ=255)                                  ttGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTc                                         >  1:190920/1‑81 (MQ=255)                                   tGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCg                                        >  2:190918/1‑81 (MQ=255)                                     cAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCt                                      <  2:190881/81‑1 (MQ=255)                                      aGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTg                                     <  2:190882/81‑1 (MQ=255)                                       gTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGt                                    <  1:190883/81‑1 (MQ=255)                                       gTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGt                                    >  2:190916/1‑81 (MQ=255)                                        tCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGtt                                   <  1:190884/81‑1 (MQ=255)                                          cGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGc                                 >  2:190929/1‑81 (MQ=255)                                          cGCCAATAAACATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGc                                 <  2:190885/81‑1 (MQ=255)                                                  aaCATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAgg                         <  2:190886/81‑1 (MQ=255)                                                    cATCGTTCTTTTCCCCTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGAt                       <  1:190887/81‑1 (MQ=255)                                                                 cccTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCAcc           <  1:190888/80‑1 (MQ=255)                                                                   cTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACcttc        <  2:190889/81‑1 (MQ=255)                                                                   cTGATATTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACcttc        <  1:190890/81‑1 (MQ=255)                                                                      atatTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACcttctt      <  2:190891/80‑1 (MQ=255)                                                                      atatTCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACcttctt      <  2:190892/80‑1 (MQ=255)                                                                          tCAAACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATc  >  1:190926/1‑80 (MQ=255)                                                                             |                                                                             GATTTCCTGATGTGGAATTTCCCATTTTGCGCCTTGCAGTCCGCCAATAAACATCGTTCTTTTCCCCTGATATTCACACAGGGTAAACGTGATCTCTGCCAGAGGAATACCTTCGCTGTTGCGGAACAGGATTGTGCTGTCACCTTCTTTATC  >  NC_000913/918482‑918634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑