Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	922,079	T→G	L578R (CTG→CGG)	macB →	ABC‑type tripartite efflux pump ATP binding/membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	922,079	0	T	G	100.0%	100.8 / NA	30	L578R (CTG→CGG)	macB	ABC‑type tripartite efflux pump ATP binding/membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/18);  total (12/18)

CGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTT  >  NC_000913/922003‑922151                                                                             |                                                                         cgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGtt                                                                      <  2:191542/81‑1 (MQ=255) cgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGtt                                                                      <  1:191541/81‑1 (MQ=255) cgGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGtt                                                                      >  1:191570/1‑81 (MQ=255)  gggAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGttt                                                                     <  1:191543/81‑1 (MQ=255)    gAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGc                                                                   <  1:191544/81‑1 (MQ=255)          ggCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTc                                                             >  1:191572/1‑81 (MQ=255)           gCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTc                                                             <  2:191545/80‑1 (MQ=255)             aTTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGt                                                          <  1:191546/81‑1 (MQ=255)                 gCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGgc                                                       <  1:191547/80‑1 (MQ=255)                  cATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGgcgc                                                     >  1:191568/1‑81 (MQ=255)                      gCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGtt                                                  <  1:191548/80‑1 (MQ=255)                          tAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGa                                              <  1:191549/80‑1 (MQ=255)                            ggTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAAt                                            <  1:191551/80‑1 (MQ=255)                            ggTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATa                                           <  2:191550/81‑1 (MQ=255)                               gcgcGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAaca                                        >  1:191587/1‑81 (MQ=255)                                 gcgAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcact                                      >  1:191576/1‑81 (MQ=255)                                   gAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgt                                    >  1:191574/1‑81 (MQ=255)                                     gCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtca                                  >  1:191577/1‑81 (MQ=255)                                       aaGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAAcactgtcact                                <  2:191552/81‑1 (MQ=255)                                               ttttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAAtt                         <  1:191553/80‑1 (MQ=255)                                                 ttGCAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGc                       <  1:191554/80‑1 (MQ=255)                                                    cAACAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCttt                    <  2:191555/80‑1 (MQ=255)                                                       cAGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCAcc                <  2:191556/81‑1 (MQ=255)                                                        aGTTCCTGATCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCt               >  2:191569/1‑81 (MQ=255)                                                             cTGGTCGAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAg          <  2:191557/81‑1 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc    >  1:191614/1‑81 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc    >  1:191579/1‑81 (MQ=255)                                                                   gAAGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTc    >  2:191588/1‑81 (MQ=255)                                                                    aaGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCt   >  1:191590/1‑81 (MQ=255)                                                                     aGCCGTACGGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCtt  <  2:191558/81‑1 (MQ=255)                                                                             |                                                                         CGGGAAATTGGCATTCGCATGGCTGTAGGTGCGCGAGCAAGCGATGTTTTGCAACAGTTCCTGATCGAAGCCGTACTGGTTTGCCTGGTCGGTGGCGCGTTGGGAATAACACTGTCACTGTTAATTGCTTTCACCTTGCAGCTTTTCTT  >  NC_000913/922003‑922151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑