Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	923,356	A→G	L31L (TTA→TTG)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	923,356	0	A	G	100.0%	105.4 / NA	31	L31L (TTA→TTG)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

ACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGG  >  NC_000913/923278‑923432                                                                               |                                                                             aCTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCt                                                                            <  2:191715/81‑1 (MQ=255) aCTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCt                                                                            >  1:191769/1‑81 (MQ=255)      aaCTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGgcgc                                                                       >  2:191749/1‑81 (MQ=255)       aCTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCgct                                                                      <  2:191716/81‑1 (MQ=255)           aGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCgctgct                                                                   >  1:191753/1‑80 (MQ=255)           aGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCgctgct                                                                   >  1:191763/1‑80 (MQ=255)           aGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCgctgc                                                                    <  1:191717/79‑1 (MQ=255)            gTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCa                                                                 <  1:191718/81‑1 (MQ=255)               tAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGt                                                               >  2:191768/1‑80 (MQ=255)                 aaTATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTaa                                                             <  2:191719/80‑1 (MQ=255)                       gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCt                                                      >  1:191756/1‑81 (MQ=255)                       gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCt                                                      <  2:191720/81‑1 (MQ=255)                            cGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCg                                                 >  2:191760/1‑81 (MQ=255)                              ccAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCggg                                               <  2:191721/81‑1 (MQ=255)                               cAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGa                                              <  1:191722/81‑1 (MQ=255)                                 gagCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAgg                                            >  2:191771/1‑81 (MQ=255)                                     gcgcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCt                                        >  1:191765/1‑81 (MQ=255)                                     gcgcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCt                                        <  1:191723/81‑1 (MQ=255)                                       gcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTg                                       >  1:191761/1‑80 (MQ=255)                                         gAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTgg                                      >  1:191758/1‑79 (MQ=255)                                         gAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGaa                                    <  1:191724/81‑1 (MQ=255)                                          aGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAg                                   >  2:191755/1‑81 (MQ=255)                                            cACCGTCATGAGTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCg                                 >  1:191778/1‑81 (MQ=255)                                                       gTTTATGACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGa                      <  2:191725/81‑1 (MQ=255)                                                             gACCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGt                >  2:191784/1‑81 (MQ=255)                                                              aCCGTCGAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGtt               <  2:191726/81‑1 (MQ=255)                                                                    gAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTc         >  1:191777/1‑81 (MQ=255)                                                                    gAGCACTTGTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTc         >  1:191766/1‑81 (MQ=255)                                                                            gTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAgg  <  2:191727/80‑1 (MQ=255)                                                                            gTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAgg  >  1:191767/1‑80 (MQ=255)                                                                            gTTGCTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAgg  >  2:191770/1‑80 (MQ=255)                                                                               |                                                                             ACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGG  >  NC_000913/923278‑923432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑